TY - JOUR T1 - Approximate Bayesian bisulphite sequencing analysis JF - bioRxiv DO - 10.1101/041715 SP - 041715 AU - Owen J.L. Rackham AU - Sarah R. Langley AU - Thomas Oates AU - Eleni Vradi AU - Nathan Harmston AU - Prashant K. Srivastava AU - Jacques Behmoaras AU - Petros Dellaportas AU - Leonardo Bottolo AU - Enrico Petretto Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/02/29/041715.abstract N2 - We present a Bayesian smoothing approach to detect differentially methylated regions from whole-genome bisulfite sequencing (WGBS) data. The method exploits the Integrated Nested Laplace Approximation for fast and accurate model fitting, an alternative to computationally expensive sampling-based methods. We demonstrate the approach by extensive simulation studies and WGBS of macrophages in experimental glomerulonephritis, revealing differential Ifitm3 promoter methylation in glomerulonephritis, supported by differential transcription factor binding and Ifitm3 gene expression. ER -