TY - JOUR T1 - Quantitative comparison of single-cell sequencing methods using hippocampal neurons JF - bioRxiv DO - 10.1101/004366 SP - 004366 AU - Luwen Ning AU - Guan Wang AU - Zhoufang Li AU - Wen Hu AU - Qingming Hou AU - Yin Tong AU - Meng Zhang AU - Li Qin AU - Xiaoping Chen AU - Hengye Man AU - Pinghua Liu AU - Jiankui He Y1 - 2014/01/01 UR - http://biorxiv.org/content/early/2014/04/21/004366.abstract N2 - Single-cell genomic analysis has grown rapidly in recent years and will find widespread applications in various fields of biology, including cancer biology, development, immunology, pre-implantation genetic diagnosis, and neurobiology. In this study, we amplified genomic DNA from individual hippocampal neurons using one of three single-cell DNA amplification methods (multiple annealing and looping-based amplification cycles (MALBAC), multiple displacement amplification (MDA), and GenomePlex whole genome amplification (WGA4)). We then systematically evaluated the genome coverage, GC-bias, reproducibility, and copy number variations among individual neurons. Our results showed that single-cell genome sequencing results obtained from the MALBAC and WGA4 methods are highly reproducible and have a high success rate. Chromosome-level and subchromosomal-level copy number variations among individual neurons can be detected. ER -