RT Journal Article
SR Electronic
T1 Generation of mutation hotspots in ageing bacterial colonies
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 041525
DO 10.1101/041525
A1 Agnieszka Sekowska
A1 Sofie Wendel
A1 Morten H. H. Nørholm
A1 Antoine Danchin
YR 2016
UL http://biorxiv.org/content/early/2016/02/26/041525.abstract
AB How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adaptation: most mutations were located in just a few hotspots in the genome and over time, mutations increasingly originated from 8-oxo-guanosine, formed exclusively on the transcribed strand.