TY - JOUR T1 - Generation of mutation hotspots in ageing bacterial colonies JF - bioRxiv DO - 10.1101/041525 SP - 041525 AU - Agnieszka Sekowska AU - Sofie Wendel AU - Morten H. H. Nørholm AU - Antoine Danchin Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/02/26/041525.abstract N2 - How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adaptation: most mutations were located in just a few hotspots in the genome and over time, mutations increasingly originated from 8-oxo-guanosine, formed exclusively on the transcribed strand. ER -