PT  - JOURNAL ARTICLE
AU  - Agnieszka Sekowska
AU  - Sofie Wendel
AU  - Morten H. H. Nørholm
AU  - Antoine Danchin
TI  - Generation of mutation hotspots in ageing bacterial colonies
AID  - 10.1101/041525
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 041525
4099  - http://biorxiv.org/content/early/2016/02/26/041525.short
4100  - http://biorxiv.org/content/early/2016/02/26/041525.full
AB  - How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adaptation: most mutations were located in just a few hotspots in the genome and over time, mutations increasingly originated from 8-oxo-guanosine, formed exclusively on the transcribed strand.