RT Journal Article
SR Electronic
T1 Persisting fetal clonotypes influence the structure and overlap of adult human T cell receptor repertoires
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 039297
DO 10.1101/039297
A1 Mikhail V. Pogorelyy
A1 Yuval Elhanati
A1 Quentin Marcou
A1 Anastasia L. Sycheva
A1 Ekaterina A. Komech
A1 Vadim I. Nazarov
A1 Olga V. Britanova
A1 Dmitriy M. Chudakov
A1 Ilgar Z. Mamedov
A1 Yuri B. Lebedev
A1 Thierry Mora
A1 Aleksandra M. Walczak
YR 2016
UL http://biorxiv.org/content/early/2016/02/09/039297.abstract
AB The diversity of T-cell receptors recognizing foreign pathogens is generated through a highly stochastic recombination process, making the independent production of the same sequence rare. Yet unrelated individuals do share receptors, which together constitute a “public” repertoire of abundant clonotypes. The TCR repertoire is initially formed prenatally, when the enzyme inserting random nucleotides is downregulated, producing a limited diversity subset. By statistically analyzing deep sequencing T-cell repertoire data from twins, unrelated individuals of various ages, and cord blood, we show that T-cell clones generated before birth persist and maintain high abundances in adult organisms for decades, slowly decaying with age. Our results suggest that large, low-diversity public clones are created during pregnancy, and survive over long periods, providing the basis of the public repertoire.