RT Journal Article
SR Electronic
T1 Bioanalyzer chips can be used interchangeably for many analyses of DNA or RNA
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 039040
DO 10.1101/039040
A1 Jessica Davies
A1 Tom Denyer
A1 James Hadfield
YR 2016
UL http://biorxiv.org/content/early/2016/02/07/039040.abstract
AB The Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA) enables small-scale gel electrophoretic separation of nucleic acids on a microfluidic chip. Shortage of chips and excess reagents is a common issue. This report explored the compatibility of two commonly used Bioanalyzer reagents with three Bioanalyzer chip types. Microfluidic electrophoretic separation of DNA and RNA using DNA High Sensitivity and RNA 6000 Nano reagents, respectively, was successfully performed on multiple chip types, following the assay-specific protocol. For RNA quality and next-generation sequencing library size estimation, the Bioanalyzer chips tested can be used interchangeably. These findings will be valuable for any laboratory using the Agilent Bioanalyzer in a shared facility.