RT Journal Article SR Electronic T1 Common methods for fecal sample storage in field studies yield consistent signatures of individual identity in microbiome sequencing data JF bioRxiv FD Cold Spring Harbor Laboratory SP 038844 DO 10.1101/038844 A1 Ran Blekhman A1 Karen Tang A1 Elizabeth A. Archie A1 Luis B. Barreiro A1 Zachary P. Johnson A1 Mark E. Wilson A1 Jordan Kohn A1 Michael L. Yuan A1 Laurence Gesquiere A1 Laura E. Grieneisen A1 Jenny Tung YR 2016 UL http://biorxiv.org/content/early/2016/02/04/038844.abstract AB Field studies of wild vertebrates are frequently associated with extensive collections of banked fecal samples, which are often collected from known individuals and sometimes also sampled longitudinally across time. Such collections represent unique resources for understanding ecological, behavioral, and phylogenetic effects on the gut microbiome, especially for species of particular conservation concern. However, we do not understand whether sample storage methods confound the ability to investigate interindividual variation in gut microbiome profiles. This uncertainty arises in part because comparisons across storage methods to date generally include only a few (≤5) individuals, or analyze pooled samples. Here, we used n=52 samples from 13 rhesus macaque individuals to compare immediate freezing, the gold standard of preservation, to three methods commonly used in vertebrate field studies: storage in ethanol, lyophilization following ethanol storage, and storage in RNAlater. We found that the signature of individual identity consistently outweighed storage effects: alpha diversity and beta diversity measures were significantly correlated across methods, and while samples often clustered by donor, they never clustered by storage method. Provided that all analyzed samples are stored the same way, banked fecal samples therefore appear highly suitable for investigating variation in gut microbiota. Our results open the door to a much-expanded perspective on variation in the gut microbiome across species and ecological contexts.