PT  - JOURNAL ARTICLE
AU  - Matthew Loose
AU  - Sunir Malla
AU  - Michael Stout
TI  - Real time selective sequencing using nanopore technology
AID  - 10.1101/038760
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 038760
4099  - http://biorxiv.org/content/early/2016/02/03/038760.short
4100  - http://biorxiv.org/content/early/2016/02/03/038760.full
AB  - The Oxford Nanopore MinION is a portable real time sequencing device which functions by sensing the change in current flow through a nanopore as DNA passes through it. These current values can be streamed in real time from individual nanopores as DNA molecules traverse them. Furthermore, the technology enables individual DNA molecules to be rejected on demand by reversing the voltage across specific channels. In theory, combining these features enables selection of individual DNA molecules for sequencing from a pool, an approach called ‘Read Until’. Here we apply dynamic time warping to match short query current traces to references, demonstrating selection of specific regions of small genomes, individual amplicons from a group of targets, or normalisation of amplicons in a set. This is the first demonstration of direct selection of specific DNA molecules in real time whilst sequencing on any device and enables many novel uses for the MinION.