TY - JOUR T1 - Human splicing diversity across the Sequence Read Archive JF - bioRxiv DO - 10.1101/038224 SP - 038224 AU - Abhinav Nellore AU - Andrew E. Jaffe AU - Jean-Philippe Fortin AU - José Alquicira-Hernández AU - Leonardo Collado-Torres AU - Siruo Wang AU - Robert A. Phillips III AU - Nishika Karbhari AU - Kasper D. Hansen AU - Ben Langmead AU - Jeffrey T. Leek Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/01/29/038224.abstract N2 - We aligned 21,504 publicly available Illumina-sequenced human RNA-seq samples from the Sequence Read Archive (SRA) to the human genome and compared detected exon-exon junctions with junctions in several recent gene annotations. 56,865 junctions (18.6%) found in at least 1,000 samples were not annotated, and their expression associated with tissue type. Newer samples contributed few novel well-supported junctions, with 96.1% of junctions detected in at least 20 reads across samples present in samples before 2013. Junction data is compiled into a resource called intropolis available at http://intropolis.rail.bio. We discuss an application of this resource to cancer involving a recently validated isoform of the ALK gene. ER -