TY - JOUR T1 - On the Importance of Hydrodynamic Interactions in the Stepping Kinetics of Kinesin JF - bioRxiv DO - 10.1101/037408 SP - 037408 AU - Yonathan Goldtzvik AU - Zhechun Zhang AU - D. Thirumalai Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/01/28/037408.abstract N2 - Conventional kinesin walks by a hand-over-hand mechanism on the microtubule (MT) by taking ~ 8nm discrete steps, and consumes one ATP molecule per step. The time needed to complete a single step is on the order of twenty microseconds. We show, using simulations of a coarse-grained model of the complex containing the two motor heads, the MT, and the coiled coil that in order to obtain quantitative agreement with experiments for the stepping kinetics hydrodynamic interactions (HI) have to be included. In simulations without hydrodynamic interactions spanning nearly twenty μs not a single step was completed in hundred trajectories. In sharp contrast, nearly 14% of the steps reached the target binding site within 6μs when HI were included. Somewhat surprisingly, there are qualitative differences in the diffusion pathways in simulations with and without HI. The extent of movement of the trailing head of kinesin on the MT during the diffusion stage of stepping is considerably greater in simulations with HI than in those without HI. Our results suggest that inclusion of HI is crucial in the accurate description of motility of other motors as well. ER -