%0 Journal Article %A FC Ingleby %A CL Webster %A TM Pennell %A I Flis %A EH Morrow %T Sex-biased gene expression in Drosophila melanogaster is constrained by ontogeny and genetic architecture %D 2016 %R 10.1101/034728 %J bioRxiv %P 034728 %X Sexual dimorphism is predicted to be constrained by the underlying genetic architecture shared between the sexes and through ontogeny, but whole-transcriptome data for both sexes across genotypes and developmental stages are lacking. Within a quantitative genetic framework, we sequenced RNA from Drosophila melanogaster at different developmental stages to examine sex-biased gene expression and how selection acts upon it. We found evidence that gene expression is constrained by both univariate and multivariate shared genetic variation between genes, sexes and developmental stages, but may be resolved by differential splicing. These results provide a comprehensive picture of how conflict over sexual dimorphism varies through development and clarifies the conditions under which it is predicted to evolve. %U https://www.biorxiv.org/content/biorxiv/early/2016/01/28/034728.full.pdf