RT Journal Article
SR Electronic
T1 Droplets Bumpers as Mechanical Sensors for Cell Migration Under Confinement
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 037275
DO 10.1101/037275
A1 D. Molino
A1 S. Quignard
A1 C. Gruget
A1 F. Pincet
A1 Y. Chen
A1 M. Piel
A1 J. Fattaccioli
YR 2016
UL http://biorxiv.org/content/early/2016/01/27/037275.abstract
AB Mechanical cell forces play determinant roles in cell growth and tissue development. Cell migration is a relevant context to explore single cell forces, since mechanotransduction and cell morphology are dynamically and biochemically co-regulated in order to modulate cell shape changes minute to minute. In this work we present the design of a hybrid microdevice made from a set of parallel PDMS microchannels in which oil emulsion droplets are introduced to be used as biocompatible and deformable cell forces transducers upon HL-60 human leukemia cell line migration. We show that the mechanical stress exerted by the cells can be measured from the analysis of the droplets deformations at the single cell level. Finally we show that acto-myosin contraction plays a role in the cell ability to cross obstacles in confinement conditions.