TY - JOUR T1 - INC-Seq: Accurate single molecule reads using nanopore sequencing JF - bioRxiv DO - 10.1101/038042 SP - 038042 AU - Chenhao Li AU - Kern Rei Chng AU - Esther Jia Hui Boey AU - Amanda Hui Qi Ng AU - Andreas Wilm AU - Niranjan Nagarajan Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/01/27/038042.abstract N2 - Nanopore sequencing provides a rapid, cheap and portable real-time sequencing platform with the potential to revolutionize genomics. Several applications, including RNA-seq, haplotype sequencing and 16S sequencing, are however limited by its relatively high single read error rate (>10%). We present INC-Seq (Intramolecular-ligated Nanopore Consensus Sequencing) as a strategy for obtaining long and accurate nanopore reads starting with low input DNA. Applying INC-Seq for 16S rRNA based bacterial profiling generated full-length amplicon sequences with median accuracy >97%. INC-Seq reads enable accurate species-level classification, identification of species at 0.1% abundance and robust quantification of relative abundances, providing a cheap and effective approach for pathogen detection and microbiome profiling on the MinION system. ER -