RT Journal Article
SR Electronic
T1 INC-Seq: Accurate single molecule reads using nanopore sequencing
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 038042
DO 10.1101/038042
A1 Chenhao Li
A1 Kern Rei Chng
A1 Esther Jia Hui Boey
A1 Amanda Hui Qi Ng
A1 Andreas Wilm
A1 Niranjan Nagarajan
YR 2016
UL http://biorxiv.org/content/early/2016/01/27/038042.abstract
AB Nanopore sequencing provides a rapid, cheap and portable real-time sequencing platform with the potential to revolutionize genomics. Several applications, including RNA-seq, haplotype sequencing and 16S sequencing, are however limited by its relatively high single read error rate (>10%). We present INC-Seq (Intramolecular-ligated Nanopore Consensus Sequencing) as a strategy for obtaining long and accurate nanopore reads starting with low input DNA. Applying INC-Seq for 16S rRNA based bacterial profiling generated full-length amplicon sequences with median accuracy >97%. INC-Seq reads enable accurate species-level classification, identification of species at 0.1% abundance and robust quantification of relative abundances, providing a cheap and effective approach for pathogen detection and microbiome profiling on the MinION system.