TY - JOUR T1 - Sensitive whole mount <em>in situ</em> localization of small RNAs in plants JF - bioRxiv DO - 10.1101/037978 SP - 037978 AU - Mouli Ghosh Dastidar AU - Magdalena Mosiolek AU - Michael D. Nodine AU - Alexis Maizel Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/01/26/037978.abstract N2 - Small regulatory RNAs are pivotal regulators of gene expression and play important roles in many plant processes. Although our knowledge of their biogenesis and mode of action has significantly progressed, we comparatively still know little about their biological functions. In particular, knowledge about their spatiotemporal patterns of expression rely on either indirect detection by use of reporter constructs or labor-intensive direct detection by in situ hybridization on sectioned material. None of the current approaches allows for a systematic investigation of small RNAs expression patterns.Here, we present a method for the sensitive in situ detection of micro-and siRNAs in intact plant tissues that utilizes both double-labelled probes and a specific cross linker. We determined the expression patterns of several small RNAs in plant roots and embryos. ER -