TY - JOUR T1 - Spatially-constrained growth enhances conversional meltdown JF - bioRxiv DO - 10.1101/027292 SP - 027292 AU - Maxim O. Lavrentovich AU - Mary E. Wahl AU - David R. Nelson AU - Andrew W. Murray Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/01/25/027292.abstract N2 - Cells that mutate or commit to a specialized function (differentiate) often undergo conversions that are effectively irreversible. Slowed growth of converted cells can act as a form of selection, balancing unidirectional conversion to maintain both cell types at a steady-state ratio. However, when one-way conversion is insuffciently counterbalanced by selection, the original cell type will ultimately be lost, often with negative impacts on the population’s overall ftness. The critical balance between selection and conversion needed for preservation of unconverted cells and the steady-state ratio between cell types depends on the spatial circumstances under which cells proliferate. We present experimental data on a yeast strain engineered to undergo irreversible conversion: this synthetic system permits cell type-specifc fuorescent labeling and exogenous variation of the relative growth and conversion rates. We fnd that populations confned to grow on a fat agar surface are more susceptible than their well-mixed counterparts to ftness loss via a conversion-induced “meltdown.” We then present analytical predictions for growth in several biologically-relevant geometries – well-mixed liquid media, radially-expanding two-dimensional colonies, and linear fronts in two dimensions – by employing analogies to the directed percolation transition from non-equilibrium statistical physics. These simplifed theories are consistent with the experimental results. ER -