RT Journal Article SR Electronic T1 When Wavelengths Collide: Bias in Cell Abundance Measurements due to Expressed Fluorescent Proteins JF bioRxiv FD Cold Spring Harbor Laboratory SP 037010 DO 10.1101/037010 A1 Ariel Hecht A1 Drew Endy A1 Marc Salit A1 Matthew S. Munson YR 2016 UL http://biorxiv.org/content/early/2016/01/22/037010.abstract AB The abundance of bacteria in liquid culture is commonly inferred by measuring optical density at 600 nm. Red fluorescent proteins (RFP) can strongly absorb light at 600 nm. Increasing RFP expression can falsely inflate apparent cell density and lead to underestimations of mean per-cell fluorescence by up to 10%. Measuring optical density at 700 nm would allow estimation of cell abundance unaffected by the presence of nearly all fluorescent proteins.