TY - JOUR T1 - Analysis of Two-State Folding Using Parabolic Approximation II: Temperature-Dependence JF - bioRxiv DO - 10.1101/037341 SP - 037341 AU - Robert S. Sade Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/01/19/037341.abstract N2 - Equations that govern the temperature-dependence of the rate constants, Gibbs energies, enthalpies, entropies and heat capacities of activation for folding and unfolding of spontaneously-folding fixed two-state systems have been derived using a procedure that treats the denatured and the native conformers as being confined to harmonic Gibbs energy wells. The notion that a two-state system is physically defined only for a set temperature range is introduced. The implications of this novel treatment for protein folding are discussed. ER -