PT  - JOURNAL ARTICLE
AU  - Robert S. Sade
TI  - Analysis of Two-State Folding Using Parabolic Approximation II: Temperature-Dependence
AID  - 10.1101/037341
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 037341
4099  - http://biorxiv.org/content/early/2016/01/19/037341.short
4100  - http://biorxiv.org/content/early/2016/01/19/037341.full
AB  - Equations that govern the temperature-dependence of the rate constants, Gibbs energies, enthalpies, entropies and heat capacities of activation for folding and unfolding of spontaneously-folding fixed two-state systems have been derived using a procedure that treats the denatured and the native conformers as being confined to harmonic Gibbs energy wells. The notion that a two-state system is physically defined only for a set temperature range is introduced. The implications of this novel treatment for protein folding are discussed.