Abstract
Microglia have fundamental roles in health and disease, however effects of age, sex and genetic factors on human microglia have not been fully explored. We applied bulk and single cell approaches to comprehensively characterize human microglia transcriptomes and their associations with age, sex and APOE. We identified a novel microglial signature, characterized its expression in bulk data from 1,306 brain samples across 6 regions and in single cell microglia transcriptome. We discovered microglial co-expression network modules associated with age, sex and APOE-ε4 that are enriched for lipid and carbohydrate metabolism genes. Integrated analyses of modules with single cell transcriptomes revealed significant overlap between age-associated module genes and both pro-inflammatory and disease-associated microglial clusters. These modules and clusters harbor known neurodegenerative disease genes including APOE, PLCG2 and BIN1. These data represent a well-characterized human microglial transcriptome resource; and highlight age, sex and APOE-related microglial immunometabolism perturbations with potential relevance in neurodegeneration.
Competing Interest Statement
The authors have declared no competing interest.
Abbreviations
- AD
- Alzheimer’s disease
- APOE
- Apolipoprotein
- EBM
- Brodmann’s area
- BSA
- Bovine serum albumin
- CERAD
- Consortium to Establish a Registry for Alzheimer’s Disease
- CNS
- Central nervous system
- CQN
- Conditional quantile normalization
- DAM
- Disease-associated microglia
- DPBS
- Dulbecco’s phosphate buffered saline
- FACS
- Fluorescence-activated cell sorting
- FPKM
- Fragments per kilobase of transcript per million mapped reads
- GEM
- Gel bead-in emulsion
- GO
- Gene ontology
- MACS
- Magnetic-activated cell sorting
- ME
- Module eigengenes
- MM
- Module membership
- PBS
- Phosphate buffered saline
- PC
- Principal component
- PCA
- Principle component analysis
- PFA
- Paraformaldehyde
- QC
- Quality control
- RNAseq
- RNA sequencing
- ROSMAP
- Rush University Religious Order Study-Memory and Aging Project
- scRNAseq
- Single cell RNA sequencing
- snRNAseq
- Single nuclei RNA sequencing
- UMI
- Unique molecular identifier
- WGCNA
- Weighted gene co-expression network analysis