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Potent neutralizing antibodies in the sera of convalescent COVID-19 patients are directed against conserved linear epitopes on the SARS-CoV-2 spike protein
Chek Meng Poh, View ORCID ProfileGuillaume Carissimo, Bei Wang, Siti Naqiah Amrun, Cheryl Yi-Pin Lee, Rhonda Sin-Ling Chee, Nicholas Kim-Wah Yeo, Wen-Hsin Lee, Yee-Sin Leo, Mark I-Cheng Chen, Seow-Yen Tan, Louis Yi Ann Chai, Shirin Kalimuddin, Siew-Yee Thien, Barnaby Edward Young, David C. Lye, Cheng-I Wang, Laurent Renia, Lisa F.P. Ng
doi: https://doi.org/10.1101/2020.03.30.015461
Chek Meng Poh
1Singapore Immunology Network, Agency of Science, Technology and Research, Immunos, Biopolis, 138648, Singapore
Guillaume Carissimo
1Singapore Immunology Network, Agency of Science, Technology and Research, Immunos, Biopolis, 138648, Singapore
Bei Wang
1Singapore Immunology Network, Agency of Science, Technology and Research, Immunos, Biopolis, 138648, Singapore
Siti Naqiah Amrun
1Singapore Immunology Network, Agency of Science, Technology and Research, Immunos, Biopolis, 138648, Singapore
Cheryl Yi-Pin Lee
1Singapore Immunology Network, Agency of Science, Technology and Research, Immunos, Biopolis, 138648, Singapore
Rhonda Sin-Ling Chee
1Singapore Immunology Network, Agency of Science, Technology and Research, Immunos, Biopolis, 138648, Singapore
Nicholas Kim-Wah Yeo
1Singapore Immunology Network, Agency of Science, Technology and Research, Immunos, Biopolis, 138648, Singapore
Wen-Hsin Lee
1Singapore Immunology Network, Agency of Science, Technology and Research, Immunos, Biopolis, 138648, Singapore
Yee-Sin Leo
2National Centre for Infectious Diseases, 16 Jalan Tan Tock Seng, 308442, Singapore
3Department of Infectious Diseases, Tan Tock Seng Hospital, 11 Jalan Tan Tock Seng, 308433, Singapore
4Lee Kong Chian School of Medicine, Nanyang Technological University, 11 Mandalay Road, 308232, Singapore
5Yong Loo Lin School of Medicine, National University of Singapore and National University Health System, 10 Medical Drive, 117597, Singapore
Mark I-Cheng Chen
2National Centre for Infectious Diseases, 16 Jalan Tan Tock Seng, 308442, Singapore
6Saw Swee Hock School of Public Health, National University of Singapore and National University Health System, 12 Science Drive 2, #10-01, 117549, Singapore
Seow-Yen Tan
7Department of Infectious Diseases, Changi General Hospital, 2 Simei Street 3, 529889, Singapore
Louis Yi Ann Chai
3Department of Infectious Diseases, Tan Tock Seng Hospital, 11 Jalan Tan Tock Seng, 308433, Singapore
8Department of Medicine, National University Hospital, 5 Lower Kent Ridge Road, 119074, Singapore
Shirin Kalimuddin
9Department of Infectious Diseases, Singapore General Hospital, 31 Third Hospital Ave, #03-03 Bowyer Block C, 168753, Singapore
10Emerging Infectious Disease Program, Duke-NUS Medical School, 8 College Road, 169857, Singapore
Siew-Yee Thien
9Department of Infectious Diseases, Singapore General Hospital, 31 Third Hospital Ave, #03-03 Bowyer Block C, 168753, Singapore
Barnaby Edward Young
2National Centre for Infectious Diseases, 16 Jalan Tan Tock Seng, 308442, Singapore
3Department of Infectious Diseases, Tan Tock Seng Hospital, 11 Jalan Tan Tock Seng, 308433, Singapore
4Lee Kong Chian School of Medicine, Nanyang Technological University, 11 Mandalay Road, 308232, Singapore
David C. Lye
2National Centre for Infectious Diseases, 16 Jalan Tan Tock Seng, 308442, Singapore
3Department of Infectious Diseases, Tan Tock Seng Hospital, 11 Jalan Tan Tock Seng, 308433, Singapore
4Lee Kong Chian School of Medicine, Nanyang Technological University, 11 Mandalay Road, 308232, Singapore
5Yong Loo Lin School of Medicine, National University of Singapore and National University Health System, 10 Medical Drive, 117597, Singapore
Cheng-I Wang
1Singapore Immunology Network, Agency of Science, Technology and Research, Immunos, Biopolis, 138648, Singapore
Laurent Renia
1Singapore Immunology Network, Agency of Science, Technology and Research, Immunos, Biopolis, 138648, Singapore
Lisa F.P. Ng
1Singapore Immunology Network, Agency of Science, Technology and Research, Immunos, Biopolis, 138648, Singapore
11Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 8 Medical Drive, 117596, Singapore
12Institute of Infection and Global Health, University of Liverpool, Liverpool, 8 West Derby Street, Liverpool L7 3EA, United Kingdom
Abstract
The ongoing SARS-CoV-2 pandemic demands rapid identification of immunogenic targets for the design of efficient vaccines and serological detection tools. In this report, using pools of overlapping linear peptides and functional assays, we present two immunodominant regions on the spike glycoprotein that were highly recognized by neutralizing antibodies in the sera of COVID-19 convalescent patients. One is highly specific to SARS-CoV-2, and the other is a potential pan-coronavirus target.
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Posted March 31, 2020.
Potent neutralizing antibodies in the sera of convalescent COVID-19 patients are directed against conserved linear epitopes on the SARS-CoV-2 spike protein
Chek Meng Poh, Guillaume Carissimo, Bei Wang, Siti Naqiah Amrun, Cheryl Yi-Pin Lee, Rhonda Sin-Ling Chee, Nicholas Kim-Wah Yeo, Wen-Hsin Lee, Yee-Sin Leo, Mark I-Cheng Chen, Seow-Yen Tan, Louis Yi Ann Chai, Shirin Kalimuddin, Siew-Yee Thien, Barnaby Edward Young, David C. Lye, Cheng-I Wang, Laurent Renia, Lisa F.P. Ng
bioRxiv 2020.03.30.015461; doi: https://doi.org/10.1101/2020.03.30.015461
Potent neutralizing antibodies in the sera of convalescent COVID-19 patients are directed against conserved linear epitopes on the SARS-CoV-2 spike protein
Chek Meng Poh, Guillaume Carissimo, Bei Wang, Siti Naqiah Amrun, Cheryl Yi-Pin Lee, Rhonda Sin-Ling Chee, Nicholas Kim-Wah Yeo, Wen-Hsin Lee, Yee-Sin Leo, Mark I-Cheng Chen, Seow-Yen Tan, Louis Yi Ann Chai, Shirin Kalimuddin, Siew-Yee Thien, Barnaby Edward Young, David C. Lye, Cheng-I Wang, Laurent Renia, Lisa F.P. Ng
bioRxiv 2020.03.30.015461; doi: https://doi.org/10.1101/2020.03.30.015461
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