Abstract
Deep-sequencing of virus isolates using short-read sequencing technologies is problematic because the viruses are often present in populations with high sequence similarity. We present a new method for generating single-read, full-length virus genomes by combining an improved Random Circular Amplification-based virus enrichment protocol with Single Molecule Real Timesequencing and a new sequence de-concatenation method. We demonstrate CIDER-Seq by producing more than 250 full-length geminivirus genomes from symptomatic field-grown plants.
Copyright
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