Abstract
Reprogramming of somatic cells into Induced pluripotent stem cells (iPSCs) by defined factors has been well established. iPSCs were shown to be very similar to Embryonic Stem Cells (ESCs) but recent studies have reported that early stage iPSCs from various somatic cell sources differ in their differentiation potency into other cell-types. However, it was noted that such iPSCs have differentiation bias to their donor cell types than to any other cell-types. Epigenetic memory in terms of DNA methylation/histone methylation of iPSCs has been attributed to the observed phenomenon but other mechanisms underlying this process remain to be explored. In the light of cell-type specific specialized ribosomes, by reanalyzing the publically available gene expression datasets among ESCs and various sources of iPSCs, here we report that transcripts of Ribosomal Protein (RP) subunit composition differ between ESCs and iPSCs. Further, ribosome protein subunit transcripts among various iPSCs are quite different and are dynamic. We studied the dynamic patterns of RP gene expression during different stages of pluripotency. We also discussed the possible outcomes/effects of deriving various cell types from these iPSC, in the context of ribosomopathies, and potential ways to overcome them. Our results provide a informatics′ framework for researchers working on deriving iPSC and the implications will have profound impact on regenerative medicine and iPSC derived patient specific cell transplantation.
List of Abreviations
- iPSCs
- Induced Pluripotent Stem Cells
- ESCs
- Embryonic Stem Cells
- RP
- Ribosomal Protein
- OSKM
- Oct4, Sox2, Klf4 and cMyc
- HDF
- Human dermal fibroblasts
- HA
- Human astrocytes
- NHBE
- Normal human bronchial epithelium
- PREC
- Human prostate epithelial cell
- hADFs
- human Adult Dermal Fibroblasts
- DBA
- Diamond Blackfan Anemia