Sequence logo is a powerful tool for presenting consensus sequences or motifs of nucleic acids and proteins (Schneider and Stephens 1990). WebLogo, a web-based sequence logo generator hosted by the University of California, Berkeley is the most popular logo generator so far (Crooks et al. 2004). WebLogo has a graphical interface and is convenient and highly configurable. However, its application is occasionally restricted by the internet speed, especially in developing countries. Moreover, when the sequence number exceeds 10,000, a command line interface will have to be used instead of graphical interface, but many users in biological sciences fields found it difficult to perform the installation and configuration of WebLogo and GhostScript (for vector map output) due to lacking relative knowledge. Here I made an application, DNAlogo, which creates DNA sequence logos in Windows with a graphical interface. The operation of DNAlogo doesn't need any knowledge on programming or bioinformatics.