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High-throughput CRISPRi phenotyping in Streptococcus pneumoniae identifies new essential genes involved in cell wall synthesis and competence development
View ORCID ProfileXue Liu, View ORCID ProfileClement Gallay, View ORCID ProfileMorten Kjos, View ORCID ProfileArnau Domenech, View ORCID ProfileJelle Slager, Sebastiaan P. van Kessel, Kèvin Knoops, Robin A. Sorg, View ORCID ProfileJing-Ren Zhang, View ORCID ProfileJan-Willem Veening
doi: https://doi.org/10.1101/088336
Xue Liu
1Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, Centre for Synthetic Biology, University of Groningen, Groningen, The Netherlands
2Center for Infectious Disease Research, School of Medicine, Tsinghua University, Beijing, China
Clement Gallay
1Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, Centre for Synthetic Biology, University of Groningen, Groningen, The Netherlands
Morten Kjos
1Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, Centre for Synthetic Biology, University of Groningen, Groningen, The Netherlands
3Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, N-1432 Ås, Norway
Arnau Domenech
1Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, Centre for Synthetic Biology, University of Groningen, Groningen, The Netherlands
Jelle Slager
1Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, Centre for Synthetic Biology, University of Groningen, Groningen, The Netherlands
Sebastiaan P. van Kessel
1Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, Centre for Synthetic Biology, University of Groningen, Groningen, The Netherlands
Kèvin Knoops
4Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands
Robin A. Sorg
1Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, Centre for Synthetic Biology, University of Groningen, Groningen, The Netherlands
Jing-Ren Zhang
2Center for Infectious Disease Research, School of Medicine, Tsinghua University, Beijing, China
Jan-Willem Veening
1Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, Centre for Synthetic Biology, University of Groningen, Groningen, The Netherlands
5Department of Fundamental Microbiology, Faculty of Biology and Medicine, University of Lausanne, Biophore Building, CH-1015 Lausanne, Switzerland
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Posted November 17, 2016.
High-throughput CRISPRi phenotyping in Streptococcus pneumoniae identifies new essential genes involved in cell wall synthesis and competence development
Xue Liu, Clement Gallay, Morten Kjos, Arnau Domenech, Jelle Slager, Sebastiaan P. van Kessel, Kèvin Knoops, Robin A. Sorg, Jing-Ren Zhang, Jan-Willem Veening
bioRxiv 088336; doi: https://doi.org/10.1101/088336
High-throughput CRISPRi phenotyping in Streptococcus pneumoniae identifies new essential genes involved in cell wall synthesis and competence development
Xue Liu, Clement Gallay, Morten Kjos, Arnau Domenech, Jelle Slager, Sebastiaan P. van Kessel, Kèvin Knoops, Robin A. Sorg, Jing-Ren Zhang, Jan-Willem Veening
bioRxiv 088336; doi: https://doi.org/10.1101/088336
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