Mobile elements (MEs) collectively constituted to at least 51% of the human genome. Due to their past incremental accumulation and ongoing DNA transposition of members from certain subfamilies, MEs serve as a significant source for both inter- and intra-species genetic diversity during primate and human evolution. Since MEs can exert direct impact on gene function via a plethora of mechanism, it is believed that the ME-derived genetic diversity has contributed to the phenotypic differences between human and non-human primates, as well as among human populations and individuals. To define the specific contribution of MEs in making Human sapiens as a genetically unique species in comparison to other primates, we aim to compile a complete list of MEs that are only uniquely present in the human genome, i.e., human specific MEs (HS-MEs). By making use of the most recent reference genome sequences for human and many other primates and a more robust and integrative multi-way comparative genomic approach, we identified a total of 15,463 HS-MEs. This list of HS-MEs represents a 120% increase from prior studies with over 8,000 being newly identified as HS-MEs. Collectively, these ~15,000 HS-MEIs have contributed to a total of 15 million base pair (Mbp) sequence increase through insertion, generation of target site duplications, and transductions, as well as a 0.5 Mbp sequence loss via insertion-mediated deletions, leading to a net total of 14.5 Mbp genome size increase. The availability of this comprehensive list of HS-MEIs permitted us to re-examine many important questions more accurately and gain new insights. New observations made with these HS-MEs include: 1) identification of several additional MEI subfamilies with significant transposition activities not visible with prior smaller datasets (e.g. L1HS, L1PA2, and HERV-K); 2) A clear similarity of the retrotransposition mechanism among L1, Alus, and SVAs that is distinct from HERVs based on the pre-integration site sequence motifs; 3) Y-chromosome as a strikingly hot target for HS-MEIs, particularly for LTRs, which showed an insertion rate 15 times higher than the genome average; 4) among the ME types, SVAs seem to show a very strong bias in inserting into existing SVAs. Among the HS-MEIs, more than 8,000 locate into the vicinity of ~4900 unique genes, in regions including CDS, untranslated exon regions, promoters, and introns of protein coding genes, as well as promoters and exons of non-coding RNAs. Such HS-MEIs have the opportunities to impact the function of their host genes via participation in protein coding, alternative splicing, and regulation at both the transcription and post-transcription levels. In seven cases, five being SVAs, MEs participate in protein coding. In conclusion, our results indicate that the amount of mobile elements uniquely occurred in the human genome is much higher than previously known, and we predict that the same is true regarding their impact on human genome evolution and function. The comprehensive list of human-specific mobile elements provides an important reference dataset for studying the impact of DNA transposition in human genome evolution and gene function.