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- October 13, 2016.
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Author Information
- Damian Kao1,
- Alvina G. Lai1,
- Evangelia Stamataki2,
- Silvana Rosic3,4,
- Nikolaos Konstantinides5,
- Erin Jarvis6,
- Alessia Di Donfrancesco1,
- Natalia Pouchkina-Stantcheva1,
- Marie Sémon5,
- Marco Grillo5,
- Heather Bruce6,
- Suyash Kumar2,
- Igor Siwanowicz2,
- Andy Le2,
- Andrew Lemire2,
- Michael B. Eisen7,
- Cassandra Extavour8,
- William E. Browne9,
- Carsten Wolff10,
- Michalis Averof5,
- Nipam H. Patel6,
- Peter Sarkies3,4,
- Anastasios Pavlopoulos2 and
- A. Aziz Aboobaker1
- 1Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, United Kingdom
- 2Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, United States
- 3MRC Clinical Sciences Centre, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London W12 0NN
- 4Institute for Clinical Sciences, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London, W12 0NN
- 5Institut de Génomique Fonctionnelle de Lyon, Centre National de la Recherche Scientifique (CNRS) and École Normale Supérieure de Lyon, Lyon, France
- 6University of California, Berkeley, Dept. of Molecular and Cell Biology, 519A LSA 3200 Berkeley, CA 94720-3200
- 7Howard Hughes Medical Institute, University of California, Berkeley, Department Of Molecular and Cell Biology
- 8Department of Organismic and Evolutionary Biology, Harvard University, 16 Divinity Avenue, BioLabs Building 4109-4111 Cambridge, MA 02138
- 9Department of Invertebrate Zoology, Smithsonian National Museum of Natural History, MRC-163, P.O. Box 37012, Washington, DC 20013-7012 USA
- 10Humboldt-Universität zu Berlin, Institut für Biologie, Vergleichende Zoologie, Philippstr. 13, Haus 2, 10115 Berlin, Germany
- For correspondence Aziz.Aboobaker{at}zoo.ox.ac.uk (AAA) pavlopoulosa{at}janelia.hhmi.org (AP)
AUTHOR INFORMATION These authors contributed equally to this work: Damian Kao, Alvina G. Lai, Evangelia Stamataki. These authors also contributed equally: Anastasios Pavlopoulos, A. Aziz Aboobaker
AUTHORS CONTRIBUTIONS Damian Kao, devised assembly strategy. Assembled and analyzed the sequencing data. Annotated the genome, transcriptome and proteome. Performed orthology group analysis. Annotated small RNAs.
Drafting and revising the article.
Alvina Lai, analysed the genome including major signaling pathways, polymorphisms, immunity, lignocellulose digestion, epigenetic pathways, small RNA pathways and small RNAs. Cloning of DSCAM variants. Experimental confirmation of polymorphisms. Drafting and revising the article.
Evangelia Stamataki, prepared the genomic libraries, performed CRISPR knock-out, performed CRISPR knock-in. Drafting revising the article.
Silvana Rosic, contributed bisulfite sequencing data and analysis of genome wide methylation.
Nikolaos Konstantinides, contributed transcriptome data and transcriptome assembly.
Erin Jarvis, performed in situ hybridization detection of Hox genes, and interpreted data.
Alessia Di DonFrancesco, contributed to confirmation of polymorphism and cloning of Ph-DSCAM variants.
Natalia Pouchkina-Stancheva, contributed to confirmation of polymorphism and cloning of Ph-DSCAM variants.
Marie Sémon, contributed transcriptome data and transcriptome assembly.
Marco Grillo, contributed transcriptome data and transcriptome assembly.
Heather Bruce, contributed transcriptome data.
Suyash Kumar, performed CRISPR knock-out.
Igor Siwanowicz, performed Parhyale cuticle staining.
Andy Le, performed CRISPR knock-in.
Andrew Lemire, was consulted about sequencing strategy and helped with bioinformatics.
Michael Eisen, Contributed to RNAseq data production.
Cassandra Extavour, Contributed to project planning.
William E. Browne, Established the Chicago-F line.
Carsten Wolff, Performed karyotyping.
Michalis Averof, contributed transcriptome data and transcriptome assembly.
Nipam Patel, performed in situ hybridization detection of Hox genes, and interpreted data. Contributed transcriptome data. Established the Chicago-F line.
Peter Sarkies, conceived and designed bisulfite sequencing experiments, contributed bisultfite sequencing data and analysis of genome wide methylation.
Anastasios Pavlopoulos, conceived, designed and managed the project. Contributed to data acquisition and analysis. Drafting and revising the article.
Aziz Aboobaker, Devised assembly strategy. Contributed to data analysis. Conceived, designed and managed the project. Drafting and revising the article.
All authors read and approved the manuscript.