Efficient collective migration depends on a balance between contractility and cytoskeletal rearrangements, adhesion, and mechanical cell-cell communication, which are all controlled by GTPases of the RHO family. Here, we report the results of a comprehensive screen assessing the contributions of the guanine nucleotide exchange factors (GEFs) to the regulation of this process during the wound healing response of a scratched human bronchial epithelial cell monolayer. Our studies uncovered GEFs that are required for collective motility at large, such as SOS1 and β-PIX, and RHOA GEFs ARHGEF18, ARHGEF11, ARHGEF3 and ARHGEF28 that are implicated in intercellular communication. Downregulation of these GEFs differentially enhance front-to-back propagation of guidance cues through the cell monolayer. These effects are partially mirrored by downregulation of RHOA expression and myosin-II activity. We conclude that for effective collective migration the RHOA-GEFs/RHOA/actomyosin pathway must be optimally tuned to compromise between generation of motility forces and restriction of intercellular communication.