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High-throughput biochemical profiling reveals Cas9 off-target binding and unbinding heterogeneity
View ORCID ProfileEvan A. Boyle, Johan O. L. Andreasson, Lauren M. Chircus, Samuel H. Sternberg, View ORCID ProfileMichelle J. Wu, Chantal K. Guegler, Jennifer A. Doudna, William J. Greenleaf
doi: https://doi.org/10.1101/059782
Evan A. Boyle
1Department of Genetics, Stanford University, Stanford, CA
Johan O. L. Andreasson
1Department of Genetics, Stanford University, Stanford, CA
2Department of Biochemistry, Stanford University, Stanford CA
Lauren M. Chircus
3Department of Chemical & Systems Biology, Stanford University, Stanford CA
Samuel H. Sternberg
4Department of Chemistry, University of California, Berkeley, CA.
Michelle J. Wu
5Biomedical Informatics Training Program, Stanford University, Stanford CA.
Chantal K. Guegler
4Department of Chemistry, University of California, Berkeley, CA.
Jennifer A. Doudna
4Department of Chemistry, University of California, Berkeley, CA.
6Department of Molecular and Cell Biology, Howard Hughes Medical Institute University of California, Berkeley, CA. Innovative Genomics Initiative, University of California, Berkeley, CA. Lawrence Berkeley National Laboratory, Berkeley CA
William J. Greenleaf
1Department of Genetics, Stanford University, Stanford, CA
7Department of Applied Physics, Stanford University, Stanford CA.
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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Posted June 19, 2016.
High-throughput biochemical profiling reveals Cas9 off-target binding and unbinding heterogeneity
Evan A. Boyle, Johan O. L. Andreasson, Lauren M. Chircus, Samuel H. Sternberg, Michelle J. Wu, Chantal K. Guegler, Jennifer A. Doudna, William J. Greenleaf
bioRxiv 059782; doi: https://doi.org/10.1101/059782
High-throughput biochemical profiling reveals Cas9 off-target binding and unbinding heterogeneity
Evan A. Boyle, Johan O. L. Andreasson, Lauren M. Chircus, Samuel H. Sternberg, Michelle J. Wu, Chantal K. Guegler, Jennifer A. Doudna, William J. Greenleaf
bioRxiv 059782; doi: https://doi.org/10.1101/059782
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