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History
- June 7, 2016.
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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
Author Information
- Kaspar Podgorski and
- Gayathri Ranganathan
New and Noteworthy New optical tools are transforming neuroscience, allowing increasingly comprehensive control and readout of neural circuits. However, emerging parallel microscopy methods rely on higher total illumination powers than conventional techniques, raising concerns about heating and its effects on the brain. This study characterizes these effects under serial and parallel illumination schemes, allowing researchers to predict power limits for their experiments. Even under serial illumination, usable power can be limited by heating rather than nonlinear damage.