Abstract
The significance of single-cell transcription resides not only in the cumulative expression strength of the cell population but also in its heterogeneity. We propose a new model that improves the detection of changes in the transcriptional heterogeneity pattern of RNA-Seq data using two heterogeneity parameters: ‘burst proportion’ and ‘burst magnitude’, whose changes are validated using RNA-FISH. Transcriptional ‘co-bursting’ – governed by distinct mechanisms during myoblast proliferation and differentiation – is described here.
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