ABSTRACT
The field of epitranscriptomics has undergone an enormous expansion in the last few years; however, a major limitation is the lack of generic methods to map RNA modifications transcriptome-wide. Here we show that using Oxford Nanopore Technologies, N6-methyladenosine (m6A) RNA modifications can be detected with high accuracy, in the form of systematic errors and decreased base-calling qualities. Our results open new avenues to investigate the universe of RNA modifications with single nucleotide resolution, in individual RNA molecules.
LIST OF ABBREVIATIONS
- (m6A)
- N6-methlyadenosine
- (m1A)
- N1-methyladenosine
- (5hmc)
- 5-hydroxymethylcytosine
- (ONT)
- Oxford Nanopore Technologies
- (SVM)
- Support Vector Machine
- (IVT)
- In vitro transcription
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.