Abstract
After invasion, Toxoplasma resides in a parasitophorous vacuole (PV) that is surrounded by the PV membrane (PVM). Once inside the PV, tachyzoites secrete dense granule proteins (GRAs) of which some, such as GRA16 and GRA24, are transported beyond the PVM likely via a putative translocon. However, once tachyzoites convert into bradyzoites within cysts, it is not known if secreted GRAs can traffic beyond the cyst wall membrane. We used the tetracycline inducible system to drive expression of HA epitope tagged GRA16 and GRA24 after inducing stage conversion and show that these proteins are not secreted beyond the cyst wall membrane.
Abbreviations
- Tet-on
- tetracycline inducible system
- TetR
- tetracycline repressor protein
- ATc
- anhydrotetracycline
- HA
- hemagglutinin epitope tag
- YFP
- yellow fluorescent protein
- Ble
- phleomycin
- HFF
- human foreskin fibroblast
- DBA lectin
- dolichos biflorus agglutinin
- IFA
- indirect immunofluorescence
- GRA
- dense granule proteins
- ROP
- rhoptry proteins
- IST
- T. gondii inhibitor of STAT1 transcriptional activity
- PV
- parasitophorous vacuole
- PVM
- PV membrane
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