Abstract
Meiotic “point recombination” refers to homologue recombination events affecting only individual SNPs. Driven mostly by gene conversion, it is common process that allows for a gradual adaptation and maturation of haplotypes during genetic evolution. In contrast to crossover recombination it is not tied to predetermined recombination sites and therefore assumed to occur largely randomly. Our analysis of archaic human haplotypes however revealed striking differences in the local point recombination rate. A linkage-study of 1.9 million SNPs defined by the sequence of denisovan hominids revealed low rates in introns and quiescent intergenic regions but high rates in splice sites, exons, 5’- and 3’-UTRs, and CpG islands. Correlations with ChIP-Seq tracks from ENCODE and other public sources identified a number of epigenetic modifications, that associated directly with these recombination events. A particularly tight association was observed for 5-hydroxymethylcytosine marks (5hmC). The mark was enriched in virtually all of the functional regions associated with elevated point recombination rates, including CpG islands and ‘poised’ bivalent regions. As intermediate of oxidative demethylation, 5hmC is also a marker of recently opened gene loci. The data, thus, supports a model of ‘guided’ evolution, in which point recombination is directed by 5hmC marks towards the functionally relevant regions.
List of abbreviations
- 5hmC
- 5-hydroxymethylcytosine
- 5mC
- 5-methylcytosine
- BivFlank
- Bivalent flanking region
- bp
- Base-pairs
- CGI
- CpG Islands
- ChIP-seq
- Chromatin Immunoprecipitation and Sequencing
- CO
- Crossover
- DDR
- DNA damage repair
- Denisovan h
- Denisovan hominid
- DMC1
- Meiotic recombination protein, RecA homolog
- ENCODE
- Encyclopedia of DNA elements
- EnhBiv
- Bivalent enhancer
- fa
- Absolute frequency of derived alleles
- FitCons
- Fitness consequence
- GWAVA
- Genome-wide annotation of variants
- H. sapiens
- Homo sapiens
- hap
- Core haplotype
- HLA
- Human Leukocyte Antigen
- kb
- Kilo-basepairs
- LD
- Linkage disequilibrium
- LWK
- Luhya from Webuye, Kenya
- MHC
- Major Histocompatibility Complex
- NCO
- Non-Crossover
- PRC2
- Polycomb repressive complex 2
- ReprPC
- Polycomb Repressed Regions
- ReprPCWk
- Weak Polycomb Repressed Regions
- SNP
- Single Nucleotide Polymorphism
- TEI
- Transgenerational Epigenetic Inheritence
- TSS
- Transcriptional start site
- TssBiv
- Bivalent transcriptional start site
- Tx
- Transcribed Region
- TxFlank
- Flanking a transcribed region
- TxWk
- Weakly transcribed region
- UTR
- Untranslated Region
- ZNF
- Zinc Finger