Abstract
Förster resonance energy transfer (FRET) using pulsed illumination has been pivotal in probing complex single molecule dynamics within subcellular environments. However, there are still major challenges in quantitative single photon, single molecule FRET (smFRET) data analysis under pulsed illumination including: 1) simultaneously deducing kinetics and number of system states; 2) providing uncertainties over estimates, particularly uncertainty over state numbers; 3) taking into account all experimental details such as crosstalk and instrument response function contributing to uncertainty; in addition to 4) background. Here, we implement the Bayesian nonparametric framework described in the first companion paper that addresses all the aforementioned issues in smFRET data analysis specialized for the case of pulsed illumination. Furthermore, we apply our method to both synthetic as well as experimental data acquired using Holliday junction.
Competing Interest Statement
The authors have declared no competing interest.