Abstract
Heparin-binding protein (HBP), as a granule protein secreted by polymorphonuclear neutrophils (PMNs) participates in the pathophysiological process of sepsis. It has been reported that HBP is a biomarker of sepsis, which is related to the severity of septic shock and organ dysfunction. HBP binds to vascular endothelial cells as one of the primary target sites. However, it is still unclear whether HBP-binding protein receptors exist on the surface of ECs. The effect of HBP on vascular permeability in sepsis and its mechanism needs to be explored. We conducted in vivo and in vitro study. We demonstrated that HBP bound to transforming growth factor-β receptor type 2 (TGF-β-R2) as a ligand. GST pull-down analysis reveals that HBP mainly interacts with the extracellular domain of TGF-β-R2. HBP induced acute lung injury (ALI) and vascular leakage via activation of TGF-β/SMAD2/3 signaling pathway. Permeability assay suggests TGF-β-R2 is necessary for HBP-induced increased permeability. We also defined the role of HBP and its potential membrane receptor TGF-β-R2 in the blood-gas barrier in the pathogenesis of HBP-related ALI.
Abbreviations
- HBP
- Heparin-binding protein
- TGF-β-R2
- Transforming growth factor-β receptor type 2
- EMT
- Epithelial–mesenchymal transition
- HUVEC
- Human umbilical endothelial cell
- PMN
- Polymorphonuclear neutrophil
- SEM
- Scanning Electron Microscope
- TEM
- Transmission Electron Microscope
- ZO
- zonula occludens
- GAPDH
- glyceraldehyde-3-phosphate dehydrogenase
- GST
- Glutathione S-transferase.