ABSTRACT
Background and Rationale Hepatoblastoma (HB) is the most common pediatric liver cancer. Its predominant occurrence in very young children led us to investigating whether the neonatal liver provides a protumorigenic niche to HB development.
Methods HB development was compared between orthotopic transplantation models established in postnatal day 5 and 60 mice (P5Tx and P60Tx models). Single-cell RNA-sequencing was performed using tumor and liver tissues from both models and the top candidate cell types and genes identified are investigated for their roles in HB cell growth, migration, and survival.
Results We found that various HB cell lines including HepG2 cells were consistently and considerably more tumorigenic and metastatic in the P5Tx model than in the P60Tx models. Sc-RNAseq of the P5Tx and P60Tx HepG2 models revealed that the P5Tx tumor was more hypoxic and had a larger number of activated hepatic stellate cells (aHSCs) in the tumor-surrounding liver which express significantly higher levels of Cxcl1 than those from the P60Tx model. We found these differences were developmentally present in normal P5 and P60 liver. We showed that the Cxcl1/Cxcr2 axis mediated HB cell migration and was critical to HB cell survival under hypoxia. Treating HepG2 P60Tx model with recombinant CXCL1 protein induced intrahepatic and pulmonary metastasis and CXCR2 knockout in HepG2 cells abolished their metastatic potential in the P5Tx model. Lastly, we showed that in metastatic HB patient tumors there was a similar larger population of aHSCs in the tumor-surrounding liver than in localized tumors, and tumor hypoxia was uniquely associated with HB patient prognosis among pediatric cancers.
Conclusion We demonstrated that the neonatal liver provides a prometastatic niche to HB development via the Cxcl1/Cxcr2 axis.
Competing Interest Statement
The authors have declared no competing interest.
Footnotes
Conflict of interest statement: The authors have declared that no conflict of interest exists.
Grant Support: This work was supported by American Cancer Society Research Scholar Grant RSG-18-026-01.
ABBREVIATIONS
- HB
- hepatoblastoma
- HSC
- hepatic stellate cells
- aHSC
- activated HSCs
- TME
- tumor microenvironment
- VLBW
- very-low-birth-weight
- sc-RNAseq
- single-cell RNA-sequencing
- PDX
- patient-derived xenograft
- ZsG
- ZsGreen
- GFP
- green fluorescent protein
- IHC
- Immunohistochemistry
- ISH
- in-situ hybridization
- CM
- conditioned media
- rhCXCL1
- recombinant human CXCL1
- KO
- knockout
- GSEA
- gene set enrichment analysis
- EMT
- epithelial-Mesenchymal Transition
- ALL
- acute lymphoblastic leukemia
- RFP
- red fluorescent protein