Abstract
Knowledge-based statistical potentials have been shown to be rather effective in protein 3-dimensional (3D) structure evaluation and prediction. Recently, several statistical potentials have been developed for RNA 3D structure evaluation, while their performances are either still at low level for the test datasets from structure prediction models or dependent on the “black-box” process through neural networks. In this work, we have developed an all-atom distance-dependent statistical potential based on residue separation for RNA 3D structure evaluation, namely rsRNASP, which is composed of short- and long-ranged potentials distinguished by residue separation. The extensive examinations against available RNA test datasets show that, rsRNASP has apparently higher performance than the existing statistical potentials for the realistic test datasets with large RNAs from structure prediction models including the newly released RNA-Puzzles dataset, and is comparable to the existing top statistical potentials for the test datasets with small RNAs or near-native decoys. Additionally, rsRNASP is also superior to RNA3DCNN, a recently developed scoring function through 3D convolutional neural networks. rsRNASP and the relevant databases are available at website https://github.com/Tan-group/rsRNASP.
SIGNIFICANCE RNAs play crucial roles in catalyzing biochemical reactions and regulating gene expression, and the biological functions of RNAs are generally coupled to their structures. Complementary to experiments, developing computational models to predict RNA 3D structures can be very helpful for understanding RNA biology functions. For a computational model, a reliable energy function is essentially important either for guiding conformational folding or for structure evaluation. For this purpose, we developed a residue-separation-based distance-dependent statistical potential, named rsRNASP which distinguishes the short- and long-ranged interactions, for RNA 3D structure evaluation. Our rsRNASP were examined against extensive test sets and shows overall superior performance over existing top traditional statistical potentials and a recently developed scoring function through 3D convolutional neural networks, especially for realistic test set from various computational structure prediction models.
Competing Interest Statement
The authors have declared no competing interest.