Abstract
Inactivation of p53 occurs in many cancers. microRNAs and RNA-binding proteins are thought to repress p53 expression through its 3’ untranslated region (3’UTR), thus contributing to tumorigenesis. We used CRISPR/Cas9 to delete the human and mouse p53 3’UTRs while preserving endogenous mRNA processing. This revealed that the endogenous 3’UTR is not involved in the regulation of p53 mRNA or protein expression, neither in steady state nor after genotoxic stress.
Competing Interest Statement
The authors have declared no competing interest.
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