Abstract
Spear-ATAC is a modified droplet-based single-cell ATAC-seq (scATAC-seq) protocol that enables simultaneous read-out of chromatin accessibility profiles and integrated sgRNA spacer sequences from thousands of individual cells at a time. Spear-ATAC profiling of 104,592 cells representing 414 sgRNA knock-down populations revealed the temporal dynamics of epigenetic responses to regulatory perturbations in cancer cells and the associations between transcription factor binding profiles, demonstrating a high-throughput method for perturbing and evaluating dynamic single-cell epigenetic states.
Competing Interest Statement
W.J.G. is a consultant for 10x Genomics who has licensed IP associated with ATAC-seq. W.J.G. has additional affiliations with Guardant Health (consultant) and Protillion Biosciences (co-founder and consultant).
Footnotes
Contact Information: William J. Greenleaf, PhD, Stanford University School of Medicine, 279 Campus Drive, Beckman Center B257, Stanford, CA 94305-5168, Email: wjg{at}stanford.edu Fax: 650-725-1534, Phone: 650-725-3672