New Results
Alpha-1 antitrypsin inhibits SARS-CoV-2 infection
Lukas Wettstein, Carina Conzelmann, Janis A. Müller, Tatjana Weil, Rüdiger Groß, Maximilian Hirschenberger, Alina Seidel, Susanne Klute, Fabian Zech, Caterina Prelli Bozzo, Nico Preising, Giorgio Fois, Robin Lochbaum, Philip Knaff, Volker Mailänder, Ludger Ständker, Dietmar Rudolf Thal, Christian Schumann, Steffen Stenger, Alexander Kleger, Günter Lochnit, Konstantin Sparrer, Frank Kirchhoff, Manfred Frick, Jan Münch
doi: https://doi.org/10.1101/2020.07.02.183764
Lukas Wettstein
1Institute of Molecular Virology, Ulm University Medical Center, Ulm, 89081, Germany
Carina Conzelmann
1Institute of Molecular Virology, Ulm University Medical Center, Ulm, 89081, Germany
Janis A. Müller
1Institute of Molecular Virology, Ulm University Medical Center, Ulm, 89081, Germany
Tatjana Weil
1Institute of Molecular Virology, Ulm University Medical Center, Ulm, 89081, Germany
Rüdiger Groß
1Institute of Molecular Virology, Ulm University Medical Center, Ulm, 89081, Germany
Maximilian Hirschenberger
1Institute of Molecular Virology, Ulm University Medical Center, Ulm, 89081, Germany
Alina Seidel
1Institute of Molecular Virology, Ulm University Medical Center, Ulm, 89081, Germany
Susanne Klute
1Institute of Molecular Virology, Ulm University Medical Center, Ulm, 89081, Germany
Fabian Zech
1Institute of Molecular Virology, Ulm University Medical Center, Ulm, 89081, Germany
Caterina Prelli Bozzo
1Institute of Molecular Virology, Ulm University Medical Center, Ulm, 89081, Germany
Nico Preising
2Core Facility Functional Peptidomics, Ulm University Medical Center, Ulm, 89081, Germany
Giorgio Fois
3Institute of General Physiology, Ulm University, Ulm, 89081, Germany
Robin Lochbaum
3Institute of General Physiology, Ulm University, Ulm, 89081, Germany
Philip Knaff
4Dermatology Clinic, University Medicine Mainz, Mainz, 55131, Germany
5Max-Planck-Institute for Polymer Research, Mainz, 55129, Germany
Volker Mailänder
4Dermatology Clinic, University Medicine Mainz, Mainz, 55131, Germany
5Max-Planck-Institute for Polymer Research, Mainz, 55129, Germany
Ludger Ständker
2Core Facility Functional Peptidomics, Ulm University Medical Center, Ulm, 89081, Germany
Dietmar Rudolf Thal
6Laboratory of Neuropathology, Department of Imaging and Pathology, KU-Leuven and Department of Pathology, UZ-Leuven, Leuven, Belgium
7Laboratory of Pathology – Institute of Pathology, Ulm University, Ulm, 89081, Germany
Christian Schumann
8Pneumology, Thoracic Oncology, Sleep and Respiratory Critical Care Medicine, Clinics Kempten-Allgäu, Kempten and Immenstadt, 87435, Germany
Steffen Stenger
9Institute for Microbiology and Hygiene, Ulm University Medical Center, Ulm, 89081, Germany
Alexander Kleger
10Department of Internal Medicine 1, Ulm University Hospital, Ulm, 89081, Germany
Günter Lochnit
11Institute of Biochemistry, Justus-Liebig University Giessen, Giessen, 35392, Germany
Konstantin Sparrer
1Institute of Molecular Virology, Ulm University Medical Center, Ulm, 89081, Germany
Frank Kirchhoff
1Institute of Molecular Virology, Ulm University Medical Center, Ulm, 89081, Germany
Manfred Frick
3Institute of General Physiology, Ulm University, Ulm, 89081, Germany
Jan Münch
1Institute of Molecular Virology, Ulm University Medical Center, Ulm, 89081, Germany
2Core Facility Functional Peptidomics, Ulm University Medical Center, Ulm, 89081, Germany
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes coronavirus disease 2019 (COVID-19). To identify factors of the respiratory tract that suppress SARS-CoV-2, we screened a peptide/protein library derived from bronchoalveolar lavage, and identified α1-antitrypsin (α1-AT) as specific inhibitor of SARS-CoV-2. α1-AT targets the viral spike protein and blocks SARS-CoV-2 infection of human airway epithelium at physiological concentrations. Our findings show that endogenous α1-AT restricts SARS-CoV-2 and repurposes α1-AT-based drugs for COVID-19 therapy.
Competing Interest Statement
The authors have declared no competing interest.
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Posted July 02, 2020.
Alpha-1 antitrypsin inhibits SARS-CoV-2 infection
Lukas Wettstein, Carina Conzelmann, Janis A. Müller, Tatjana Weil, Rüdiger Groß, Maximilian Hirschenberger, Alina Seidel, Susanne Klute, Fabian Zech, Caterina Prelli Bozzo, Nico Preising, Giorgio Fois, Robin Lochbaum, Philip Knaff, Volker Mailänder, Ludger Ständker, Dietmar Rudolf Thal, Christian Schumann, Steffen Stenger, Alexander Kleger, Günter Lochnit, Konstantin Sparrer, Frank Kirchhoff, Manfred Frick, Jan Münch
bioRxiv 2020.07.02.183764; doi: https://doi.org/10.1101/2020.07.02.183764
Alpha-1 antitrypsin inhibits SARS-CoV-2 infection
Lukas Wettstein, Carina Conzelmann, Janis A. Müller, Tatjana Weil, Rüdiger Groß, Maximilian Hirschenberger, Alina Seidel, Susanne Klute, Fabian Zech, Caterina Prelli Bozzo, Nico Preising, Giorgio Fois, Robin Lochbaum, Philip Knaff, Volker Mailänder, Ludger Ständker, Dietmar Rudolf Thal, Christian Schumann, Steffen Stenger, Alexander Kleger, Günter Lochnit, Konstantin Sparrer, Frank Kirchhoff, Manfred Frick, Jan Münch
bioRxiv 2020.07.02.183764; doi: https://doi.org/10.1101/2020.07.02.183764
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