Summary/Abstract
The GlycReSoft software tool allows users to process glycoproteomics LC-MS data sets. The tool accepts proteomics database search results or a user-defined list of proteins in the sample. GlycReSoft processes LC-MS data to yield deconvoluted exact mass values. The user has the option to import a list of theoretical glycans from an external database, a curated glycan list, or a measured glycome. The tool assembles a list of theoretical glycopeptides from the lists of theoretical glycans and proteins, respectively. The program then scores the tandem mass spectra in the LC-MS data files and provides graphical views of the identified glycopeptides for each protein in the sample, and the set of glycoforms identified for each peptide sequence.
Competing Interest Statement
The authors have declared no competing interest.
Footnotes
Contact information: Joseph Zaia, Boston University Medical Campus, 670 Albany St., Rm. 509, Boston, MA 02119, USA, (v) 1-617-638-6762, (e) jzaia{at}bu.edu