Abstract
Long chain polyunsaturated fatty acids (LCPUFA) are bioactive components of membrane phospholipids and serve as substrates for signaling molecules. LCPUFA can be obtained directly from animal foods or synthesized endogenously from 18 carbon precursors via the FADS2 coded enzyme. Vegans rely almost exclusively on endogenous synthesis to generate LCPUFA and we hypothesized that an adaptive genetic polymorphism would confer advantage. The rs66698963 polymorphism, a 22 bp insertion-deletion within FADS2, is associated with basal FADS1 expression, and coordinated induction of FADS1 and FADS2 in vitro. Here we determined rs66698963 genotype frequencies from 234 individuals of a primarily vegetarian Indian population and 311 individuals from the U.S. A much higher I/I genotype frequency was found in Indians (68%) than in the U.S. (18%). Analysis using 1000 Genomes Project data confirmed our observation, revealing a global I/I genotype of 70% in South Asians, 53% in Africans, 29% in East Asians, and 17% in Europeans. Tests based on population divergence, site frequency spectrum and long-range haplotype consistently point to positive selection encompassing rs66698963 in South Asian, African and some East Asian populations. Basal plasma phospholipid arachidonic acid status was 8% greater in I/I compared to D/D individuals. The biochemical pathway product-precursor difference, arachidonic acid minus linoleic acid, was 31% and 13% greater for I/I and I/D compared to D/D, respectively. Our study is consistent with previous in vitro data suggesting that the insertion allele enhances n-6 LCPUFA synthesis and may confer an adaptive advantage in South Asians because of the traditional plant-based diet practice.
Introduction
Twenty and twenty two carbon long chain polyunsaturated fatty acids (LCPUFA), especially arachidonic acid (ARA; 20:4n-6), eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3) are ubiquitous in mammalian tissue, are bioactive components of membrane phospholipids (PL) and serve as precursors to cell signaling eicosanoids and docosanoids that are major drug targets (e.g., COX-1, COX-2 inhibitors, leukotriene receptor antagonists) (Park, Kothapalli, Lawrence, et al. 2009; Park, Kothapalli, Reardon, et al. 2009; Park, et al. 2011). LCPUFA can be obtained directly from animal foods or endogenously synthesized from 18 carbon essential fatty acid precursors linoleic acid (LA; 18:2n-6) and alpha-linolenic acid (ALA; 18:3n-3) and their metabolites by an alternating series of desaturation and elongation reactions (Park, Kothapalli, Lawrence, et al. 2009). Vegans rely on this biochemical pathway to generate all LCPUFA from precursors. Classic carnivores (e.g. cats and most marine fish) have lost the metabolic ability to make LCPUFA and rely on consumption of animal tissue to supply all their LCPUFA requirements. Traditional human populations have been described that are analogous to herbivores (vegans) and carnivores (Natives of the Canadian Arctic) (Gibson and Sinclair 1981). The genomic determinants of the function of this pathway are therefore candidates for selective pressure based on human diets habitually consumed through many generations, e.g., by traditional vegan/vegetarian populations as are found in India. Signals of positive selection surrounding the FADS gene cluster have been reported in Greenlandic Inuit and in African populations (Ameur, et al. 2012; Mathias, et al. 2012; Fumagalli, et al. 2015).
The fatty acid desaturase genes (FADS1 [OMIM#606148] and FADS2 [OMIM#606149]) code for enzymes that catalyze the introduction of double bonds at specific positions in a fatty acid chain. FADS1 (Δ5-desaturase) and FADS2 (Δ6/Δ8/Δ4-desaturase) have specificity for several fatty acid substrates (Park, Kothapalli, Lawrence, et al. 2009; Park, et al. 2011; Park, et al. 2015; Park, et al. 2016). In humans, the FADS genes (FADS1, FADS2, and FADS3) evolved evolutionarily by gene duplication events and are clustered within the 100 kb region on the long arm of human chromosome 11 (11q12-13.1) (Marquardt, et al. 2000), a genomic cancer hot spot (Park, et al. 2011). Our previous results show extensive splicing of all three desaturases, including a novel function of an alternatively spliced isoform (Park, Kothapalli, Reardon, et al. 2009; Park, et al. 2010; Park, et al. 2012; Park, et al. 2015).
FADS2 codes for a desaturase [EC 1.14.19.3] catalyzing the rate limiting steps in the biosynthesis of LCPUFA (Park, Kothapalli, Lawrence, et al. 2009). Supplementary fig. S1 shows the n-3 and n-6 biochemical LCPUFA pathways. Genetic studies have shown common single nucleotide polymorphisms (SNPs) within FADS gene cluster are strongly associated with LCPUFA levels and disease phenotypes (Schaeffer, et al. 2006; Malerba, et al. 2008; Tanaka, et al. 2009; Illig, et al. 2010). Using lymphoblasts from Japanese HapMap participants, we identified a 10 SNP-haplotype in FADS2 (rs2727270 to rs2851682) controlling basal FADS1 mRNA expression; minor allele carriers showed lower basal FADS1 mRNA expression in cultured lymphoblasts (Reardon, et al. 2012). A conserved genomic region within the haplotype contained predicted binding sites for a sterol regulatory element binding protein (SREBP). By amplifying a 629 bp portion flanking the sterol response element (SRE), we identified a polymorphic 22 bp insertion/deletion (indel) genetic variant (rs66698963), of which the deletion is the minor allele. Minor allele homozygotes (D/D) had significantly lower expression of FADS1 than the I/I major allele homozygotes (Reardon, et al. 2012). Arachidonic acid is the immediate product of FADS1, leading directly to the hypothesis that individuals carrying D/D genotype have lower metabolic capacity to produce LCPUFA from precursors than I/I individuals.
Here we present the first experimentally determined allele and genotype frequencies for the indel rs66698963 using a primarily vegetarian population from Pune, India compared to a U.S. population drawn broadly from around the country. By using genetic variations data from the 1000 Genomes Project (1000GP), we estimated the global genotype frequency distribution of rs66698963. By applying a series of tests for recent positive selection based on population divergence, site frequency spectrum (SFS), and long-range haplotype, we provide strong evidence that positive selection drove the insertion allele to high frequency not only in Africans, but also in South and some East Asians. The association of basal arachidonic acid status and indel genotype was evaluated in a subset of the U.S. population.
Results and Discussion
Experimentally Determined Genotype and Allele Frequencies of rs66698963
In the U.S. sampling, D/D genotype was 43% (n=134), I/D 39% (n=120) and I/I 18% (n=57); in the Indian samples D/D genotype was 3% (n=7), I/D 29.5% (n=69) and I/I 67.5% (n=158) (fig. 1A). The observed genotype frequencies in the U.S. sampling deviated from the Hardy Weinberg Equilibrium (HWE) expected frequencies, whereas sampling from India were consistent with HWE. As the U.S. population includes many definable subgroups, the deviation is likely to be due to stratification (Nussbaum, et al. 2007). In U.S. sampling, the observed allele frequency was D: 0.62, I: 0.38, whereas in Indian samples it was D: 0.18, I: 0.82. As a large fraction of our US sampling (n=201) is from the Kansas cohort, we tested allele frequency separately from this cohort and found similar trends (D: 0.64, I: 0.36).
Global Genotype Frequency of rs66698963 Determined by using 1000GP Data
As our small sampling represents only two populations, we examined the global genotype frequency distribution of the rs66698963, by using the most recent whole-genome sequencing data from the 1000GP (Phase 3) (Auton, et al. 2015). The frequency of rs66698963 (indel) was retrieved from the 1000GP as the frequency of rs373263659 (indel) after we confirmed with Sanger sequencing that rs373263659 was actually a misannotation of rs66698963 (supplementary fig. S2). 1000GP data catalogs human genetic variation of 26 different global populations. Among the five populations of South Asian ancestry, D/D was found in 1.8% (n=9), I/D 28.2% (n=138), and I/I 69.9% (n=342), very consistent with observations from our experimental data from Pune, India. For the seven populations of African ancestry, D/D was 5.1% (n=34), I/D 41.5% (n=274), and I/I 53.4% (n=353). For the five populations of European ancestry, D/D was 25.0% (n=126), I/D 58.4% (n=294), and I/I 16.5% (n=83). For the five populations of East Asian ancestry, D/D was 16.3% (n=82), I/D 54.9% (n=277), and I/I 28.8% (n=145). Overall, the I/I genotype frequency is highest in African and South Asian populations, but lower in European and East Asian populations (supplementary table S1 and fig. 1B). Among the five populations of South Asian ancestry, Gujarati Indian from Houston, Texas (GIH) has the highest I/I genotype frequency, 80%, followed closely 78% by Indian Telugu from the UK (ITU). The lowest frequency of 59% is observed in Bengali from Bangladesh (BEB).
Evaluation of Positive Selection on the FADS Region Surrounding rs66698963
Dramatic genotype frequency differences of rs66698963 among continental populations, led us to check if positive selection on the FADS region surrounding the rs66698963 has taken place especially in South Asian populations. We assessed rs66698963 genotype frequency differences among continental populations using the FST statistic, which quantifies the degree of population differentiation in allele frequencies. A value of 0 indicates no differentiation and value of 1 indicates complete subdivision. Over the four continental populations (African, European, East Asian and South Asian), the FST statistic of rs66698963 is 0.121 (empirical p value = 0.039), suggesting higher population differentiation than expected by chance. To gain insight into which population has undergone unusual frequency change as a result of regional adaptation, we further calculated pairwise FST among each pair of the four continental populations, as well as population branch statistics (PBS) for each population. Significantly high FST values were observed between South Asians and Europeans (FST = 0.276, empirical p = 2.71x10−4), between South Asians and East Asians (FST = 0.168, empirical p = 0.016), and between Africans and Europeans (FST = 0.157, empirical p = 0.044). No significant divergence was observed between South Asians and Africans (FST = 0.028) or between Europeans and East Asians (FST = 0.021). Together with the PBS patterns (supplementary fig. S3), these results suggest that rs66698963 has been subjected to positive selection in more than one of the studied populations.
To further test for positive selection signals in the region surrounding rs66698963 and to identify which populations have experienced positive selection, we applied formal tests of natural selection of two general types: (i) based on site frequency spectrum (SFS); and (ii) based on haplotype length and frequency. For SFS-based tests, we calculated genetic diversity as the average pairwise number of differences in the locus (π), Tajima’s D (Tajima 1989), and Fay and Wu’s H (Fay and Wu 2000). Positive selection could result in loss of genetic diversity, an excess of rare variants, which can be detected by Tajima’s D (negative values), and an excess of high-frequency derived alleles, which can be detected by Fay and Wu’s H (negative values). We applied these tests in each of the four continental regions, combining populations within the same continental region, and in each of the five populations of South Asian origin. All tests were performed across the whole genome using a sliding-window approach with a window size of 5 kilobase (Kb) and a step size of 1 Kb. Statistical significance was assessed with the empirical genome-wide distribution. Our analysis at the continental level (fig. 2A, supplementary figs. S4-S7) confirmed a pattern observed in a previous study: positive selection signals on FADS1 was observed only in African populations, but not in European or East Asian populations (Mathias, et al. 2012). For this genomic region surrounding FADS1, the three test statistics in the South Asian populations follow a similar but less significant pattern as in African populations. For the immediate genomic region surrounding rs66698963, we observed extremely negative Fay and Wu’s H values, restricted to South Asians (fig. 2A). African populations also had lower H values in the same region, but the reduction was not significant. For the combined group of five populations of South Asian origin, three consecutive 5-Kb windows, representing chromosome 11 region 61,601,001 – 61,608,001 bp, had significant Fay and Wu’s H values, with the most significant value being −12.27 (empirical p = 0.031). For each of the five populations individually, we also observed significant H statistics surrounding rs66698963 (fig. 2B, supplementary figs. S8-S12). Two of these populations, GIH and ITU, exhibited the most significant signals of positive selection (supplementary figs. S9 and S10). The most significant H statistic in GIH was −14.20 (empirical p = 0.015) and in ITU it was −13.80 (empirical p = 0.018). The Tajima’s D statistic also showed a significant signal of positive selection for these two South Asian populations: −1.99 in GIH (empirical p = 0.013) and −1.90 in ITU (empirical p = 0.025).
For haplotype-based tests for positive selection, we calculated the integrated Haplotype Score (iHS) (Voight, et al. 2006), the number of segregating sites by length (nSL) (Ferrer-Admetlla, et al. 2014), and Cross-population Extended Haplotype Homozygosity (XPEHH) (Sabeti, et al. 2007) for all genome-wide common variants (minor allele frequency > 5%) and statistical significance of each statistic was assessed with the empirical genome-wide distribution. Among the four continental regions (fig. 3), the most significant signals of positive selection were observed in South Asians, which have an iHS value of −2.79 (empirical p = 4.7x10−3) and a nSL value of −4.38 (empirical p = 8.95x10−5), with the haplotype carrying the insertion allele showing unusually long length and high frequency (fig. 4). Significant XPEHH of rs66698963 was also observed between South Asians and Europeans with a value of 3.47 (empirical p = 1.2x10−3, fig. 5A). Taken together, these haplotype-based test results are consistent with the SFS-based tests and point to a selective sweep on the FADS region in South Asians, with the adaptive allele being the insertion of rs66698963 or a variant in very strong linkage disequilibrium (LD) with it.
In addition to South Asians, signals of positive selection were also found in Africans and East Asians (fig. 3, supplementary figs. S13-S19). For continental level analysis with populations in the same continental region combined into a single group, Africans have iHS value of −1.70 (empirical p = 0.043) and nSL value of −2.30 (empirical p = 0.017) for rs66698963 while East Asians have iHS value of −2.05 (empirical p = 0.022) and nSL value of −1.80 (empirical p = 0.045). No significant signals of positive selection were detected for Europeans in any of our tests. Consistent with population divergence results based on FST, XPEHH of rs66698963 is also significant between Africans and Europeans with a value of 1.64 (empirical p = 0.039, fig. 5B).
Finally, we performed haplotype-based analyses in each of the five East Asian populations separately because of the frequency difference of rs66698963 across them (fig. 1B) and because analysis of East Asians combined as a single group produced apparent inconsistent results: significant haplotype-based results but lack of significant divergence from Europeans. Significant iHS and nSL results of rs66698963 were only found in CHB and JPT (supplementary fig. S17), the two populations with highest insertion allele frequency (70% and 75% respectively). Taken together, strong signals of positive selection on the region surrounding rs66698963 were consistently found in South Asians, Africans and some East Asians. All results point to rs66698963 as being at the center of the signal of selection, with the adaptive allele being the insertion allele of rs66698963 or a variant in very strong LD with it.
Challenge in Indel Calling with Low-depth Sequencing Data
While our Sanger sequencing and agarose gel electrophoresis data provided accurate frequency estimates for the Indian and U.S. samples, the global frequency pattern of rs66698963 derived from the 1000GP data may be subjected to inaccurate annotation. Indel calling has been proved challenging with short-read mapping data sets and indel variants cataloging is still in its infancy (Li, et al. 2014). Due to the low sequencing coverage depth (~6 X) in the 1000GP, annotation error is possible. Using the Sanger sequencing data of rs66698963 from 16 Japanese (JPT) individuals from our previous study, which were chosen to be homozygotes at rs66698963 (Reardon, et al. 2012), we assessed the accuracy of frequency estimates from 1000GP. Based on Sanger sequencing, 11 out of 16 individuals are I/I genotype and the remaining five are D/D genotype. All eleven I/I individuals were accurately identified in 1000GP, whereas, among the five D/D individuals, only one was correctly identified, with the other four inaccurately annotated as I/D heterozygotes. This observation suggests that inaccurate allele calling in 1000GP may result in lower number of D/D individuals and higher number of I/D individuals (fig. 1B). Better sequencing data are needed in the future for accurate estimates. It is noteworthy that our above analysis of natural selection based on SFS and long-range haplotype are unlikely to be affected by the inaccuracy of rs66698963 calling, because these statistical tests draw information from many genetic variants in the surrounding region.
Interpretation of the Signals of Positive Selection Surrounding rs66698963
Our evolutionary tests based on population divergence, SFS, and long-range haplotype, demonstrated consistently strong signals of positive selection surrounding rs66698963 in South Asian, African and some East Asian populations (CHB and JPT). Positive selection on the FADS gene cluster have been reported in Africans (Ameur, et al. 2012; Mathias, et al. 2012) and in Greenlandic Inuit (Fumagalli, et al. 2015) populations. Our study is the first to demonstrate positive selection in South and some East Asian populations. Most importantly, we propose a putatively causal indel, with regulatory effects on FADS1 and FADS2 (Reardon, et al. 2012).
The signatures of positive selection in African populations previously described by Mathias et al. (Mathias, et al. 2012) were replicated in our study and further confirmed with additional evidence from haplotype-based tests (fig. 3). This region is upstream of rs66698963 and its positive selection signatures are unlinked from those immediately surrounding the indel. However, the possibility could not be ruled out that the same adaptive variant is responsible for the observed signatures of selection in both clusters. The other study suggesting positive selection on FADS genes in African populations proposed an adaptive haplotype based on 28 SNPs in the FADS region (Ameur, et al. 2012). Although no positive selection signals were found in European populations, the adaptive haplotype in Africans, referred to as haplotype D, is present in 62% of Europeans and associated with greater LCPUFA status in Europeans (Ameur, et al. 2012). To test the possibility that the adaptive and association signals of this haplotype D are the same as those of the insertion allele of rs66698963, we examined the proportion of haplotype D carrying insertion allele at rs66698963. Among all phased haplotypes from the four continental regions, 2829 carry insertion allele at rs66698963, 72.9% of them also carry the haplotype D. For 2357 haplotypes carrying haplotype D, 87.5% also carry the insertion allele (supplementary fig. S20A). To mitigate the effect of annotation error at rs66698963, we further tested the correspondence between the haplotype D defined with 28 SNPs and the major haplotype defined with 10 SNPs from our previous study (Reardon, et al. 2012). These two sets of SNPs have three in common. Based on our previous study in Japanese individuals, all 10-SNP major haplotype carry insertion allele at rs66698963 (Reardon, et al. 2012). Among 2762 chromosomes carrying the major haplotype, 85.05% also carry haplotype D, while 99.66% of 2357 chromosomes carrying haplotype D also carry the major haplotype (supplementary fig. S20B). This strong correspondence between haplotype D and our major haplotype was also observed when stratifying into each of the four continental regions (supplementary fig. S20C-F), indicating that the association and adaptive signals described in Ameur et al. (Ameur, et al. 2012) are very likely the same as we reported in this study. However, since previous evidence of positive selection was only drawn from XPEHH and a composite likelihood (CLR) test of the allele frequency spectrum (Ameur, et al. 2012), our analyses, with a series of SFS-based and haplotype-based tests, dramatically strengthen the evidence of positive selection in Africans. Most importantly, our study proposes a putatively causal variant and demonstrates that the adaptive signals are also present in South and some East Asians, with the strongest signals in South Asians.
Most recently, Fumagalli et al. described signatures of adaptation on the FADS gene cluster in indigenous people of Greenland, the Inuit, which have subsisted on a marine diet rich in omega-3 PUFA (Fumagalli, et al. 2015). Two SNPs carrying adaptive signals, rs7115739 and rs174570, were also associated with reduced LCPUFA status. The derived alleles of these two SNPs are almost fixed in the Greenlandic Inuit but have much lower frequencies in other populations (Fumagalli, et al. 2015). We observed that one of the two SNPs, rs174570, also exhibits positive selection signals in South Asians (nSL = −3.77, p = 0.00048) and East Asians (iHS = −2.41, p = 0.010; nSL = −2.41, p = 0.012). Interestingly, it is the ancestral allele of the SNP that is adaptive in these populations, in contrast to the derived allele in Greenlandic Inuit (Fumagalli, et al. 2015). The ancestral allele has strong LD with the insertion allele of rs66698963 in South and East Asians (D’ = 0.72 and 0.92, respectively). It is plausible that the ancestral allele of rs174570 may be tagging the effect of the insertion allele of rs66698963. If that is the case, our study might not only report a functional, adaptive allele (the insertion) in South Asians, some East Asians, and Africans, traditionally residing on plant-based diet, but also an adaptive allele (the deletion) in Greenlandic Inuit with marine diet rich in omega-3 fatty acids.
Functional Analysis of rs66698963 Genotypes and Fatty Acid Levels
Associations between genotype and PUFA concentration in RBC PL from the Kansas cohort were used to probe whether the I/I genotype, with putative higher endogenous arachidonic acid synthetic capacity, follow a pattern of metabolites consistent with the pathway: higher product and lower precursor in the order I/I, I/D, and D/D. RBC fatty acid levels were measured in 199 Kansas cohort subjects, of them n=89 were D/D genotype, n=76 were I/D genotype and n=34 were I/I genotype. Participant characteristics of the Kansas cohort are presented in supplementary table S2. n-6 PUFA acids were significantly and consistently related to genotype. Fig. 6 presents associations between genotype and n-6 PUFA concentration in RBC PL, a long term (several month) measure of whole body status. Linoleic acid (18:2) was not related to genotype. In contrast, the LCPUFA 20:3n-6, 20:4n-6 (arachidonic acid) and 22:4n-6 (adrenic acid) are all strongly related to genotype. All LCPUFA comparisons are significant between the D/D and I/I genotypes. 20:3n-6 levels are highest in D/D and lowest in I/I, whereas the 20:4n-6 and 22:4n-6 are opposite. The differences in PUFA concentration calculated for various precursor-product pairs shows the strongest relationship between 20:4n-6 and 18:2n-6, where I/D and I/I are both different from D/D. Other differences are not as strong but consistent with expectations from the desaturation-elongation pathway. All differences between genotypes remained significant after controlling for the effect of age.
The metabolic pathway relating these PUFA is 18:2n-6→→20:3n-6→20:4n-6→22:4n-6. The step 20:3→20:4 is mediated by FADS1, basal expression of which our previous data show is modulated by the genotype (Reardon, et al. 2012). The three steps between the 18:2n-6→→→20:4n-6 sequentially use FADS2, a usually rapid elongation system, and FADS1. Our current results are consistent with hypothesis emerging from our work in lymphoblasts that arachidonic acid would be greater for individuals with the I/I genotype compared to D/D because of basal FADS1 expression (Reardon, et al. 2012). The immediate precursor 20:3n-6 is lower, and the immediate product, 20:4n-6, is higher, following the hypothesized FADS1 expression. The precursor 18:2n-6 is not different among genotypes, pointing to the FADS1 step as the driver for the observed altered levels. Future studies with larger and more diverse samples are warranted to further evaluate the functional implications of this novel indel polymorphism.
Linoleic acid (18:2n-6) is the most prevalent PUFA in the U.S. diet, with mean intake exceeding 13 grams per day (Brenna and Lapillonne 2009; Naqvi, et al. 2012). While it is a precursor of ARA, LA lowering does not reduce circulating ARA because the overwhelming amount of diet LA delivered by seed oils, principally soy oil in the US (Blasbalg, et al. 2011), causes ARA levels to reach a plateau (Gibson, et al. 2013). For instance, reduction of LA intake from 8.5 to 4.2 g/d over 4 weeks resulted in no significant change in plasma PL ARA (9.7 to 9.9%,w/w), though DHA rose 8% (3.47 to 3.77%,w/w) (Wood, et al. 2014). Dietary preformed ARA is required to overcome the maximum and increase circulating ARA (O’Dea and Sinclair 1985). Increasing ARA is accompanied by increasing ARA-derived eicosanoid synthesis including vasoactive species (Ferretti, et al. 1997; Nelson, et al. 1997), though oral ARA consumption at least in the short term do not produce acute clinical effects. How sustained basal ARA levels influence chronic disease progression is not well understood as an isolated factor. The 8% difference in basal ARA probably reflects differences in maximal levels for any particular individual. However, GWAS evidence suggests that the FADS gene cluster is associated with diseases that may be of the complex inflammatory type, such as colorectal cancer risk (Zhang, Jia, et al. 2014) and cardiovascular disease (Zhang, Johnson, et al. 2014). The FADS gene cluster was found in association with asthma pathogenesis using eQTL mapping (Sharma, et al. 2014), and a FADS SNP was found to influence synthesis of arachidonic acid (ARA) and synthesis of pro-inflammatory lipoxygenase products (Hester, et al. 2014).
Dietary Practices and LCPUFA
Over many generations in India, approximately 35% of the population follows the traditional lacto-vegetarian diet practice (Raheja, et al. 1993; Key, et al. 2006); our Indian cohort followed this trend with responses to dietary pattern questions indicating 38% vegetarian (unpublished), congruent with our dietary instruments applied to research participants in other studies that consistently show a vegetarian dietary pattern and generally low meat consumption in this region (Gadgil, et al. 2014). As plant sources contain only the LCPUFA precursors LA and ALA, individuals on plant based diets must biosynthesize LCPUFA endogenously. The higher frequency of I/I alleles (67.5%) among Indians supports the hypothesis that insertion genotype and its associated haplotype is under selective pressure due to its higher metabolic capacity to convert precursors (LA and ALA) to LCPUFA. This I/I genotype may be favored in populations depending on vegetarian diets and possibly populations having limited access to diets rich in LCPUFA, especially fatty fish.
Homo sapiens evolved eating a diet characterized by 4:1 to 1:1 ratio of n-6/n-3 PUFA (Eaton, et al. 1998). Based on the diet of evolutionary adaptedness (DEA), the overall n-6/n-3 estimate was found to be about 0.79 (Eaton, et al. 1998), whereas, the modern Western diet has undergone an extraordinary increase in n-6 linoleic acid resulting in a ratio above 10/1, and is likely to be even higher in Indian diet, estimate to be 20-50 (Raheja, et al. 1993; Simopoulos 2002). Substantial evidence has accumulated that this dramatic shift is a risk factor for lifestyle-related diseases, such as cardiovascular, diabetes, cancer, and inflammation-related diseases (Raheja, et al. 1993; Simopoulos 2002). Most commercially produced seed oils, sunflower, safflower, peanut, grapeseed, cottonseed and corn, contain very high levels of n-6 LA; individuals with I/I genotype having higher metabolic capacity to convert precursors to longer chain PUFA may be at increased risk for proinflammatory disease states as they efficiently convert LA to ARA. Put another way, individuals with the I/I genotype may be vulnerable to ill-health when adopting a diet rich in n-6 LA which severely reduce synthesis of anti-inflammatory n-3 LCPUFA because n-6 competes with n-3 to access the Δ-6 desaturase enzyme. Moreover, n-6 PUFA compete with and antagonize against incorporation of n-3 PUFA into tissue (Alvheim, et al. 2012), challenging the metabolic requirement for structural n-3 PUFA that are especially concentrated in neural tissue (Diau, et al. 2005).
Role of Genetic Factors in the Conversion of PUFA Precursors to Products
Metabolism studied using stable isotope labeling, candidate gene SNP, GWAS and metabolomics show interindividual variation in the conversion of PUFA precursors to LCPUFA products depends on genetic factors (Emken, et al. 1994; Schaeffer, et al. 2006; Tanaka, et al. 2009; Illig, et al. 2010). An early FADS gene cluster association study found an inherited component accounting for 28% of the variance in ARA levels among minor allele carriers of a 11 SNP haplotype (Schaeffer, et al. 2006). Minor allele carriers showed lower serum phospholipid ARA. Several independent association studies replicated these findings, showed PUFA levels to be associated with genotypes (Malerba, et al. 2008; Martinelli, et al. 2008; Xie and Innis 2008; Bokor, et al. 2010; Rzehak, et al. 2010; Lattka, et al. 2011; Sergeant, et al. 2012). Our present results show product-precursor differences, arachidonic acid to linoleic acid, to be 13% and 31% greater for I/D and I/I compared to D/D, respectively. Converging data from genetic studies show SNP variants within intron 1 of FADS2 to be associated with human phenotypes such as IQ scores, blood fatty acid levels and complex diseases (Caspi, et al. 2007; Aulchenko, et al. 2009; Standl, et al. 2011; Steer, et al. 2012; Groen-Blokhuis, et al. 2013; Zhang, Jia, et al. 2014). For instance, commonly reported SNP variants (rs174575, and rs1535) which are associated with increased IQ scores, blood fatty acid levels and complex diseases are <600 bp and <5000 bp upstream from the FADS2 indel, respectively. In humans, common SNP variants are often found to follow indels (Lu, et al. 2012), suggesting the hypothesis that rs174575 and/or rs1535 are tags for the functional genomic indel that directly modulates binding at the nearby SRE.
Putative Hypothesis rs66698963 Locus Control Bidirectional Regulation
Our previous cell culture data indicating that FADS1 and FADS2 expression (mRNA) is upregulated in response to SREBP modulators suggests that this intronic region is a master switch controlling bidirectional regulation of FADS1 and FADS2, as illustrated in supplementary fig. S21. This hypothesis is analogous to regulatory control elsewhere in the human genome: a genome-wide survey of gene organization estimates 11.6% of human genes are bidirectionally oriented (Liu, et al. 2011). For instance, LXR regulated ABC transporters (ABCG5 and ABCG8), which arose by gene duplication events, are arranged in a head-to-head orientation on HSA 2p21. They are transcribed in divergent directions and share common regulatory elements (Remaley, et al. 2002). Similarly, COL4A1 and COL4A2 oriented head-to-head on HSA 13q34 also contain a bidirectional transcription unit (Pollner, et al. 1997).
Conclusions
Collectively our data suggest that FADS2 indel genotypes contribute to individual variability in response to PUFA consumption. In the vegan or vegetarian scenario in which only, or primarily, precursors would be consumed, tissue LCPUFA would depend on the relative proportions of 18:2n-6 and 18:3n-3. In a food system dominated by linoleic acid, I/I genotype carriers would maintain higher basal arachidonic acid and presumably greater inflammatory potential and attendant higher rates of chronic disease related to inflammation. Balanced consumption of precursors would be particularly important for I/I genotype carriers. I/I carriers consuming excess 18:2n-6 may particularly benefit from consumption of EPA and DHA which bypass the desaturation steps, as a direct balance to ARA. Further, D/D individuals may also benefit from EPA and DHA consumption in pregnancy and in development when neural and other tissue dependent on omega-3 LCPUFA are rapidly develop. For chronic diseases exacerbated by inflammation, D/D carriers maintain lower ARA levels and thus lower inflammatory potential. They are predicted to be less vulnerable to excess 18:2n-6 intake. Future studies should incorporate FADS2 indel screening as a potential genetic marker for studying LCPUFA regulation.
Materials and Methods
Biological Sampling Details
A total of 311 human samples (blood, breastmilk, and placenta) were obtained from Kansas City, Rochester NY, and seven breast milk banks around the U.S. and Canada. Similarly, a total of 234 human blood samples were obtained from Pune City, India. All were used for genotyping. Only RBC (Red Blood Cells) samples from Kansas City were used for fatty acid profiling.
Study Approvals and Baseline Characteristics
The study was approved by the institutional review boards on human subjects research from all participating institutions, as follows. The University of Kansas Medical Center Human Subjects Committee, University of Rochester Research Subjects Review Board, KEM Hospital Ethics Committee and Institutional Review Board of Sinhgad Institute, India, all approved human sample collection and written informed consent was obtained from all donors. The Cornell University Institutional Review Board approved collection of samples and waived consent because the samples were collected and anonymized at human milk banks (exemption 4), as follows: Mothers’ Milk Bank, San Jose, CA; Bronson Mothers’ Milk Bank, Kalamazoo, MI; Mothers’ Milk Bank, a program of Rocky Mountain Children’s Health Foundation, Denver, CO; Ohio Health Mothers’ Milk Bank, Columbus, OH; Mothers’ Milk Bank at Austin, Austin, TX; Mothers’ Milk Bank of Northeast, Newton Upper Falls, MA; British Columbia Women’s Milk Bank, Vancouver, BC, Canada. Subjects (n=234) from Indian cohort were all Asian Indians, among them n=122 were women (ages 18 to 43 years) and n=112 were men (ages 23 to 36 years). Dietary patterns from surveys (not presented) indicated that n=89 (38.03%) were vegetarians and n=145 (61.97%) were omnivores. The US cohort (311 participants) were all females. Breastmilk (n=69) samples from US cohort are banked samples. Placental (n=41) samples are from pregnant adolescents (ages ≤18 years) who were recruited from the Rochester Adolescent Maternity Program in Rochester, NY (Cao, et al. 2014). Detailed demographic characteristics of Kansas cohort (n=201) are provided elsewhere (Carlson, et al. 2013). All participants were English speaking and are between ages 16 to 36 years. RBC fatty acid analysis were available only for the Kansas City participants (n=199). Fatty acid data is not available from the Indian cohort.
Genotyping rs66698963
Genomic DNA was extracted and was used to amplify a 629 base pair fragment flanking the SRE within intron 1 of FADS2 (GenBank Accession# NT_167190.1) by PCR as previously described (Reardon, et al. 2012). The following primer pairs were used to amplify 629 bp fragment: FADS2 forward primer 5′ TTTCTCAAAGGCCGTGGTGT 3′, FADS2 reverse primer 5′ AGTGCTAACCACTCCTGGAA 3′. The amplified products were run on 2% agarose gels until the two alleles, if present, were well separated. The gels were stained with ethidium bromide and visualized under UV to identify indel genotypes. A gel demonstrating detection of the three possible genotypes for the 22 bp indel is presented in Figure 1. Samples were assigned pairs of genotypes of I (insertion) or D (deletion) as I/I, I/D, or D/D.
Genotype Frequency of rs66698963 in 1000GP
VCF files of genetic variations data from the 1000GP (phase 3) were downloaded from the official FTT site (ftp://ftp.1000genomes.ebi.ac.uk/vol1/ftp/release/20130502/). The indel rs66698963 was not directly annotated in the VCF file while another indel rs373263659 with the same length of insertion was present at the same region. Using Sanger sequencing results from our previous study (Reardon, et al. 2012), we determined that rs373263659 is a misannotation of rs66698963. To confirm this observation and to further test the accuracy of indel calling in 1000GP, we examined the read mapping results for 16 Japanese individuals, for whom we have Sanger sequencing results. BAM files for these 16 individuals were downloaded from each individual’s directory on the FTP site and visualized with SAMtools (Li, et al. 2009). After confirming that rs373263659 is a misannotation of rs66698963 and the 1000GP has acceptable calling accuracy for this indel, we used directly the frequency of rs373263659 as that of rs66698963.
In the 1000GP, there are in total 2504 individuals from five continental regions and 26 global populations. Four admixed American populations were excluded from our analysis because of their admixture history. After the exclusion, there are seven populations of African ancestry (AFR): Yoruba in Ibadan, Nigeria (YRI), Luhya in Webuye, Kenya (LWK), Gambian in Western Divisions in the Gambia (GWD), Mende in Sierra Leone (MSL), Esan in Nigeria (ESN), Americans of African Ancestry in SW USA (ASW), African Caribbeans in Barbados (ACB); five populations of European ancestry (EUR): Utah Residents with Northern and Western European Ancestry (CEU), Toscani in Italia (TSI), Finnish in Finland (FIN), British in England and Scotland (GBR), Iberian Population in Spain (IBS); five populations of East Asian ancestry (EAS): Han Chinese in Beijing, China (CHB), Japanese in Tokyo, Japan (JPT), Southern Han Chinese (CHS), Chinese Dai in Xishuangbanna, China (CDX), Kinh in Ho Chi Minh City, Vietnam (KHV); and five populations of South Asian ancestry (SAS): Gujarati Indian from Houston, Texas (GIH), Punjabi from Lahore, Pakistan (PJL), Bengali from Bangladesh (BEB), Sri Lankan Tamil from the UK (STU), Indian Telugu from the UK (ITU).
Testing for Positive Selection on the FADS Region
SFS-based statistics, including genetic diversity (TT) Tajima’s D (Tajima 1989), and Fay and Wu’s H (Fay and Wu 2000), and population differentiation-based statistics, FST (Weir and Cockerham 1984) and population branch statistics (PBS) (Yi, et al. 2010) were calculated using in-house Perl scripts, which are available upon request. FST and PBS were calculated for each genetic variant. Genetic diversity, Tajima’s D, and Fay and Wu’s H were calculated using a sliding-window approach with window size of 5 Kb and moving step of 1 Kb. Statistical significance for these statistics were assessed using the ranking of genome-wide variants or windows, respectively. For example, if the Tajima’s D value for a specific 5-Kb window is among the top 5% of all genome-wide windows, then it is considered as statistically significant with an empirical p value <0.05. PBS was estimated based on pair-wise FST values among three continental regions (Yi, et al. 2010). For a specific locus, the PBS value for a population represents the amount of allele frequency change in the history of this population since its divergence from the other two populations. The PBS estimate for a population depends on the choice of the other two populations. Therefore, to calculate PBS for South Asians, we chose three combinations of continental regions: AFR, EUR and SAS; AFR, EAS and SAS; EUR, EAS and SAS.
Haplotype-based tests for positive selection, including the Integrated Haplotype Score (iHS) (Voight, et al. 2006), the number of segregating sites by length (nSL) (Ferrer-Admetlla, et al. 2014), and Cross-population Extended Haplotype Homozygosity (XPEHH) (Sabeti, et al. 2007), were calculated using the software selscan v 1.1.0a (Szpiech and Hernandez 2014). All SNPs with minor allele frequency greater than 5% in at least one of the four continental regions were included in our calculation. Genetic variants without information on their ancestral allele were excluded from analyses. Information on ancestral allele was retrieved directly from the VCF file with an exception for the indel under study, rs66698963, whose ancestral allele was not defined in the VCF file. Alignment of homologous sequences from human, Chimpanzee and Orangutan showed that neither the insertion nor the deletion allele of rs66698963 is present in Chimpanzee or Orangutan (supplementary fig. S22A). By examining the mapping results of short sequencing reads from a Neanderthal from the Altai Mountains (Prufer, et al. 2014) and an archaic Denisovan individual (Meyer, et al. 2012) to the human genome, we found that the insertion allele is present in both Neanderthal and Denisovan genomes and it is therefore considered as the ancestral allele. Empirical p values were inferred based on the genome-wide distribution of the statistics. The haplotype bifurcation diagrams and EHH plots were drawn using an R package, rehh (Gautier and Vitalis 2012).
Fatty Acid Analysis
RBC phospholipids (PL) fatty acids were analyzed for 199 samples from Kansas according to methods reported elsewhere (Carlson, et al. 2013) and all fatty acids were correlated with genotype. Briefly, lipids from RBC were extracted according to the modified Folch method (Folch, et al. 1957). Extracted RBC lipids were fractionated by loading on to the TLC plate (Zail and Pickering 1979). Fatty acid methyl esters (FAME) from RBC PL were prepared using boron trifluoride-methanol (Morrison and Smith 1964), and were separated by using an SP-2560 capillary column (100 m; Sigma Aldrich) (Smuts, et al. 2003). Peak integration and analysis was carried out by using a Star 6.41 Chromatography Workstation. Individual peaks were identified by comparing their retention times with those of known standards (PUFA 1 and PUFA 2; Sigma Aldrich), and an equal weight FAME mixture (Supelco 37 Component FAME mix; Sigma Aldrich) was used to adjust fatty acids for area/weight to calculate a final percentage weight of total fatty acids (Carlson, et al. 2013). Fatty acids are expressed as percent weight-for-weight (%,w/w). Percent changes are expressed as Ax/x/AD/D, where A denotes a fatty acid, x = I or D, and D/D is the deletion/deletion genotype. Pairwise statistical analysis was conducted using Student’s t-test at P<0.05 and data are presented as mean ± 95% confidence interval. Secondarily, the non-parametric Wilcoxon method was also performed to verify significance in case of skewed fatty acid distributions.
Conflict of interest
The authors declare no conflict of interest.
NOTE: 1. The numbers of individuals of specific genotype are presented in parenthesis.
2. Consult methods section for populations represented by the population codes.
Acknowledgements
This work was supported by NIH grant R01 AT007003 from the National Center for Complementary and Integrative Health (NCCIH) (formerly the National Center for Complementary and Alternative Medicine (NCCAM)) and the Office of Dietary Supplements (ODS), NIH grant R01 HD047315, USDA-NIFA National Research Initiative 2005-35200-15218, and NIH grant R01 GM108805. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH, USDA, or any of their units. The authors thank Dr. R.R. Ran-Ressler, Cornell University, for assistance with breast milk samples.